以自组装法制备的银溶胶膜为表面增强拉曼散射活性基底实现了对水中抗生素的痕量检测。 采用微波加热法制备银溶胶, 自组装法制备银溶胶膜。 通过改变银溶胶的pH值及镀膜的次数, 研究其对抗生素增强效果的影响。 实验发现, 采用不同pH值的银溶胶镀膜所获得的银溶胶膜的增强效果有很大差异, 当银溶胶pH=4, 且镀膜次数为五次时, 增强效果最佳。 以此银溶胶膜为基底对三种抗生素（氯霉素、 环丙沙星、 恩诺沙星）进行了SERS检测, 可以检测到的最低浓度分别为120, 15, 120 nmol·L-1。 结果表明, 利用改进方法制备的银溶胶膜, 可以对水中抗生素进行痕量检测, 为实现养殖水中残留抗生素的检测提供了方法。

In the present paper, silver colloid films prepared by self-assembly method were used as surface-enhanced Raman scattering(SERS) active substrates to achieve trace detection of antibiotics in water. Silver colloids were prepared using the microwave heating method, and silver colloids films were prepared with self-assembly method. The enhancement of sliver colloid films to antibiotics was analysed by changing the pH value of silver colloid and times of films. Significant effects of pH value on silver colloid films were observed. And the silver colloid films with five times, prepared by silver colloids with pH 4 had the best enhancement factor. They were used as SERS active substrates to detect three antibiotics (chloramphenicol, ciprofloxacin, enrofloxacin). The experimental limits of detection were 120, 15, 120 nmol·L-1, respectively. These results show that such substrate has a very high sensitivity and application value, and might be able to be used for trace detection of antibiotics in aquiculture.