开发溶酶体荧光探针对于研究与溶酶体相关的细胞生理、病理活动具有重要的理论意义及实际应用价值。7-二乙胺基-3-(2′-吡啶)香豆素(L3)是一种弱碱性化合物, 易于累积在酸性溶酶体中, 有作为溶酶体荧光探针的潜质。本文详细研究了pH值对L3光谱性质的影响及其溶酶体定位成像能力。研究结果表明, 该化合物具有一定的溶酶体定位能力, 可以作为溶酶体荧光探针先导化合物。

The study of lysosomal fluorescence probe has important theoretical significance and practical value for cell physiological and pathological research associated with lysosome. As a weak basic compound which prefer to the acid lysosome, 7-(diethylamino)-3-(pyridin-2-yl)coumarin (L3) may be a potential lysosome -targetable fluorescent probe. The relationship between pH value and the spectroscopic properties of L3 were investigated as well as lysosome-targetable fluorescent imaging in this study. The results show that L3 can be used as a lead compounds for lysosome-targetable fluorescent probe.