Chinese Optics Letters, 2013, 11 (11): 111102, Published Online: Dec. 6, 2013
Label-free biomolecular imaging using scanning spectral interferometry Download: 540次
110.0110 Imaging systems 120.0120 Instrumentation, measurement, and metrology 300.0300 Spectroscopy 330.0330 Vision, color, and visual optics
Abstract
This letter presents a label-free biomolecular imaging technique based on white-light interferometry and spectral detection. The method measures thickness changes caused by specific binding between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can reach ~1 nm, thereby enabling detection of trace amounts (~1 ng/mm2) of proteins or DNA. This letter also presents two experiments to prove the feasibility of the method for detecting proteins and DNA without fluorescent labeling.
Tongzhou Wang, Liping Xie, Haley Huang, Xin Li, Ruliang Wang, Guang Yang, Yanan Du, Guoliang Huang. Label-free biomolecular imaging using scanning spectral interferometry[J]. Chinese Optics Letters, 2013, 11(11): 111102.