Chinese Optics Letters, 2013, 11 (11): 111102, Published Online: Dec. 6, 2013  

Label-free biomolecular imaging using scanning spectral interferometry Download: 540次

Author Affiliations
Abstract
This letter presents a label-free biomolecular imaging technique based on white-light interferometry and spectral detection. The method measures thickness changes caused by specific binding between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can reach ~1 nm, thereby enabling detection of trace amounts (~1 ng/mm2) of proteins or DNA. This letter also presents two experiments to prove the feasibility of the method for detecting proteins and DNA without fluorescent labeling.

Tongzhou Wang, Liping Xie, Haley Huang, Xin Li, Ruliang Wang, Guang Yang, Yanan Du, Guoliang Huang. Label-free biomolecular imaging using scanning spectral interferometry[J]. Chinese Optics Letters, 2013, 11(11): 111102.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!