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应用共焦空间微分显微镜获取边缘增强显微图像

Application of Spatial Differential Confocal Microscopy in Obtaining Edge Enhanced Microscopic Images

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摘要

生物样品图像边缘增强与提取是医学图像处理的关键技术之一, 是进行生物样品形态分析的基础。图像边缘增强通常是通过计算机编程对原始图像进行后期处理来实现的, 然而, 这里应用共焦空间微分显微镜系统, 实现了在获得样品共焦显微图像的同时直接获取对应的边缘增强显微图像, 且图像分辨率与对比度较高。实验不仅获取了掩膜板与标准分辨率板RTA-07的边缘增强图像, 说明系统分辨率达到1.5 μm, 而且实现了生物细胞的边缘增强图像的获取, 如血红细胞与口腔上皮细胞, 图像可用于样品尺度与面积等形态的分析与计算, 在生物医学研究中有实际应用意义。

Abstract

Edge enhancement and extraction of biological samples′ images are one of the key technologies for the processing of medical images, and the basis of samples′ morphological analysis. In general, the realization for image edge enhancement is through post processing of original images by computer programming. However, spatial differential confocal microscopy system has been applied to directly obtain the high resolution and contrast edge enhanced microscopic images of samples while the confocal microscopic images are obtained. The biomedical application is demonstrated not only for edge enhancement imaging for a mask and a resolution test target (RTA-07) that shows the resolution of the system is 1.5 μm, but also for edge enhancement imaging for biological cells like RBCs and oral epithelial cells. The obtained edge enhanced microscopic images can be used for simply sample morphological analysis and calculation, such as the scale and area, which has a practical application significance in the research of biomedicine.

Newport宣传-MKS新实验室计划
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中图分类号:O766+.1

DOI:10.3788/aos201434.0317001

所属栏目:医用光学与生物光学

基金项目:国家自然科学基金(61178086)、广东省自然科学基金重点项目(S2013020012810)

收稿日期:2013-10-21

修改稿日期:2013-11-12

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作者单位    点击查看

吴丽如:华南师范大学物理与电信工程学院, 广东 广州 510006
唐志列:华南师范大学物理与电信工程学院, 广东 广州 510006广东省量子调控工程与材料重点实验室, 广东 广州 510006
吴泳波:华南师范大学物理与电信工程学院, 广东 广州 510006
池妍:华南师范大学物理与电信工程学院, 广东 广州 510006
黄敏芳:华南师范大学物理与电信工程学院, 广东 广州 510006

联系人作者:唐志列(tangzhl@scnu.edu.cn)

备注:吴丽如(1987-), 女, 硕士研究生, 主要从事激光共聚焦显微成像和光声成像方面的研究。E-mail: forexamplewu@126.com

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