光谱学与光谱分析, 2014, 34 (2): 455, 网络出版: 2015-01-13  

荧光光谱法测定生物炭/秸秆输入土壤后酶活性的变化

Change in Soil Enzymes Activities after Adding Biochar or Straw by Fluorescent Microplate Method
作者单位
1 中国科学院沈阳应用生态研究所森林与土壤生态国家重点实验室, 辽宁 沈阳110016
2 中国科学院寒区旱区工程与环境研究所, 甘肃 兰州730009
3 东北大学理学院, 辽宁 沈阳110004
摘要
土壤α-葡萄糖苷酶、 β-葡萄糖苷酶直接参与土壤有机物质的矿化过程, 对维持生态系统碳和养分的循环起重要作用。 秸秆或者秸秆炭化物还田是提高碳储量的一种有效措施, 输入到土壤后对生物学活性产生一定影响, 对碳库转化具有直接或者间接作用。 采用荧光物质作为底物, 将96微孔板和荧光检测法结合, 利用多功能酶标仪测定生物炭/秸秆(2.5 g/50 g干土)添加条件下黑土中α(β)葡萄糖苷酶活性。 结果表明, 秸秆添加到土壤后, 土壤α(β)葡萄糖苷酶活性增强, 水稻秸秆处理β-葡萄糖苷酶活性高于玉米秸秆处理, 40天后仍然保持较强活性, 说明秸秆的输入有助于土壤碳素转化。 可能是因为秸秆炭添加提高土壤中养分含量, 促进微生物活性, 使得土壤酶活性提高, 水稻秸秆添加增强土壤酶活性, 而玉米秸秆没有促进作用, 可能与材料来源不同有关, 材料来源与添加效应的关系有待进一步研究。 而生物炭添加对黑土中α(β)葡萄糖苷酶活性影响不大, 这与生物炭含速效养分少、 自身难降解特性有关。 与传统的分光光度法相比, 微孔板荧光法可以灵敏的检测到土壤悬液中的酶活性, 可批量检测样品, 是一种快速、 准确、 简便的土壤酶活性测定方法。
Abstract
The present work was aimed to study soil α-glucosidase and β-glucosidase activities of and red soils based on fluorescence detection method combined with 96 microplates with TECAN Infinite 200 Multi-Mode Microplate Reader. We added biochar or straw (2.5 g air dry sample/50g air dry soil sample) into and red soils and the test was carried under fixed temperature and humidity condition (25 ℃, 20% soil moisture content). The results showed that straw addition enhances soil α- glucosidase and β-glucosidase activities, β-glucosidase activity stimulated by rice straw treatment was higher than that of corn straw treatment, and activity still maintains strong after 40 days, accounting for increasing soil carbon transformation with straw inputting. Straw inputting increased soil nutrients contents and may promote microbial activity, which also lead to the increase oin enzyme activities. Different effects of straw kinds may be related to material source that needs further research. However, biochar inputting has little effect on soil α- glucosidase and β- glucosidase activity. Biochar contains less available nutrients than straw and have degradation-resistant characteristics. Compared with the conventional spectrophotometric method, fluorescence microplate method is more sensitive to soil enzyme activities in suspension liquid, which can be used in a large number of samples. In brief, fluorescence microplate method is fast, accurate, and simple to determine soil enzymes activities.

张玉兰, 陈利军, 段争虎, 武志杰, 孙彩霞, 王俊宇. 荧光光谱法测定生物炭/秸秆输入土壤后酶活性的变化[J]. 光谱学与光谱分析, 2014, 34(2): 455. ZHANG Yu-lan, CHEN Li-jun, DUAN Zheng-hu, WU Zhi-jie, SUN Cai-xia, WANG Jun-yu. Change in Soil Enzymes Activities after Adding Biochar or Straw by Fluorescent Microplate Method[J]. Spectroscopy and Spectral Analysis, 2014, 34(2): 455.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!