利用叶绿素荧光技术, 对受相同浓度Cu2+胁迫的蛋白核小球藻、斜生栅藻、铜绿微囊藻的最佳暗适应时间进行研究.通过对三种供试藻在光照和暗适应时间分别为30 s、1 min、3 min、5 min、10 min和20 min条件下的光合荧光参量进行测定, 以光化学淬灭参量值为主要参考依据, 结合t检验方法对暗适应时间进行显著性差异分析, 结果表明: 暗适应条件下三种供试藻的潜在最大量子效率值略有增加, 实际量子效率值基本保持不变；蛋白核小球藻和斜生栅藻的光化学淬灭参量值和非光化学淬灭参量值随暗适应时间的延长显著增加；铜绿微囊藻光化学淬灭参量值在光照1 min时达到最大, 无需进行暗适应, 这可能与蓝藻在暗适应时发生状态转换有关；藻类不是暗适应时间越长越好, 蛋白核小球藻和斜生栅藻的最佳暗适应时间分别为5 min和10 min.这将为采用叶绿素荧光技术进一步研究毒物对藻类的胁迫机理提供可靠依据.

The best dark adaptation time of Chlorella pyrenoidosa, Snedesmus obliquus and Microcystis aeruginosa stressed by Cu2+ was investigated by chlorophyll fluorescence measurements. Photosynthetic fluorescence parameters were measured under light and dark conditions. Measurement time in light condition were 30 s, 1 min, 3 min, 5 min, 10 min and 20 min as well as in dark condition. Significant difference of dark adaptation time was analyzed mainly based on the photochemical quenching parameter and t-test method. The results show that compared to light, the potential maximum quantum efficiency values of three algaes increase slightly under dark adaptation and the yield value remaines the same; the photochemical quenching parameter value and non photochemical quenching parameter value increase significantly as time goes on; the maximum photochemical quenching parameter value of Microcystis aeruginosa achieves when the algae is treated under light in 1 min, and Microcystis aeruginosa needs no dark adaptation may because blue-green algae state conditions shifts under dark adaptation; the dark adaptation time of algaes are not better by long time treated, and the best dark adaptation time of Chlorella pyrenoidosa and Snedesmus obliquus are 5 min and 10 min respectively. All of these will provide a reliable basis for further studies on the inhibiting effect of toxicant on algaes.