表面增强拉曼光谱(surface enhanced Raman spectroscopy, SERS)具有极高的灵敏度, 是一种强大的检测低浓度分析物的痕量分析技术, 甚至可以实现单分子检测。因此, 在化学、生物、环境等领域都是非常重要的分析手段。但是, 由于SERS信号的重现性不高, 尚未成为常规的定量分析技术。本文阐述了SERS的基本原理, 总结了应用SERS实现定量检测的研究成果, 评述了SERS的定量检测在环境、生物医药、食品卫生等方面的应用, 并提出了展望和亟待解决的问题。

Surface enhanced Raman spectroscopy (SERS) which has the advantages of high specificity and sensitivity is a powerful tool for trace analysis, and its potential single-molecule sensitivity attracts much gaze of researchers. However, the reproducibility of the SERS signal is not so acceptable; SERS has not been established as a routine analytic technique. This review expounds the fundamental theory of SERS, summarizes the current status of the evolution and observation of SERS applied on quantitative assay, and makes a comment on the application of SERS on the environment, biomedicine, food hygiene and other aspects. In particular, it raises some problems to be solved, and discusses the future applications of SERS in quantitative detection.