研制了一套高速三维扫频光学相干层析(SS-OCT)成像系统。该系统基于快速扫频激光技术，轴向扫描速率可达50 KHz。为了缩短开发周期，系统软件部分采用LabVIEW和Matlab混合编程，实现了模块化设计，主要包括时序控制、数据采集、数据处理和图象重建，界面友好并方便维护。系统基于K线性时钟触发的数据采集模式，无须额外的光谱标定，可以实现实时等波数间隔的干涉光谱信号采样。实验测得系统的轴向分辨率为8.9 μm，灵敏度在整个成像深度范围保持在100 dB以上。系统可以实现二维OCT图像的实时显示和1.8 s内三维OCT图像的采集。利用研制的SS-OCT系统，成功获得了手指和苹果果皮的在体二维和三维图像。三维OCT图像可以显示出指纹特征，而在二维手指图像上很难看到。

The self-developed high-speed three-dimensional swept optical coherence tomography (SS-OCT) system is reported. Based on fast swept laser technology, the system can realize high speed axial scan (A-scan) rate of 50 KHz. In order to shorten the development cycle, the system software is based on LabVIEW combining with Matlab. Which achieves modular design including timing control, data acquisition, data processing and image reconstruction. The system has a friendly interface and is easy to maintain. It utilizes an external clock uniform in K-space as triggers synchronously generated by the swept source—a K-trigger mode for data acquisition so that a uniform data sampling in K-space is enabled without any other linear wavenumber re-calibracation. Actually measured axial resolution of the system is 8.9 μm. The systerm sensitivity is experimentally determined to be above 100 dB in the whole depth range. This SS-OCT systerm is capable of realtime display of two-dimensional OCT and can obtain three-dimensional OCT with a measurement time of 1.8 s. Vivo human finger segments and apple peel tissue are investigated two- and three-dimensionally. The three-dimensional OCT volumes chearly show the structures of the fingerprint which are difficult to be observed in two-dimensional OCT images.