为了克服传统分光光度计中棱镜和凹面光栅等分光器件的光谱分光效率低、分辨率不高和光通量小等缺点，采用平面光栅作为色散器件,采用线阵电荷耦合器件(CCD)结合外设互换(PCI)总线高速数据采集卡作为光谱检测系统，并且利用虚拟仪器技术成功研制了一款高性价比小型生化分析仪用分光光度计，同时自制了一套交叉非对称式(CT)型单色仪作为该分光光度计的分光装置。通过实验证明，该小型分光光度计可以实现紫外和可见光波段吸光强度为1000(a.u.)的探测，光谱测量范围可达300~800 nm，波长分辨率可达1 nm，波长重复性优于0.5 nm，在波长为360 nm 处的杂散光小于0.5%，波长准确度优于±0.5 nm。相比其他同类分光光度计，该分光光度计不仅具有后分光、光谱多通道并行分析等优点，同时能优化光路成像系统，提高光谱测量准确度和降低成本。

To overcome the drawbacks of prism and concave gratings commonly used as dispersive devices in the traditional spectrophotometer, including low splitting efficiency, low resolution and little luminous flux, a high performance micro-spectrophotometer for biochemical analyzer (BCA) based on charge coupled devices (CCD) and virtual instrument technique is successfully developed, in which a plane grating is employed as the dispersive device, a linear CCD and a high speed peripheral component interconnect (PCI) bus data acquisition card are combined as the spectral detection system. Meanwhile, a custom-built Czerny-Turner (CT) typed monochrometer with a symmetric crossed structure is designed as the light splitting apparatus in this spectrophotometer. The experimental results demonstrate that this spectrophotometer realizes the capacity of detecting absorption intensity larger than 1000 (a.u.) in the ultra-violet and visible wavebands, the wavelength repeatability is less than 0.5 nm, the stray-light is less than 0.5% at the wavelength of 360 nm, its spectral accuracy is less than ±0.5 nm and its spectral measurement range and resolution reaches 300~800 nm and 1 nm, respectively. In addition, this system has the characteristics of post light-splitting and multi-channel parallel analysis, etc. This spectrophotometer for BCA has the potential value by virtue of optimized imaging system, high accuracy and low cost, etc.