光子学报, 2014, 43 (9): 0930003, 网络出版: 2014-10-23
FTIR研究南方锈病侵染对玉米叶片中蛋白质和碳水化合物的影响
Influence of Protein and Carbohydrate in Maize Leaf by Infection of Maize Southern Rust Using FTIR
光谱学 傅里叶变换红外光谱 曲线拟合 蛋白质二级结构 南方锈病 Spectroscopy Fourier transform infrared spectroscopy Curve-fitting Protein secondary structure Southern rust
摘要
为深入研究南方锈病侵染玉米叶片时叶片的生化指标变化, 使用傅里叶变换红外光谱仪分别获取感染和未感染玉米叶片的光谱, 选用光谱中的特征区和指纹区吸收带分析玉米叶片中蛋白质和碳水化合物的变化情况.玉米叶片被感染后, 其光谱的酰胺带发生变化, 碳水化合物的主要吸收带变化不明显.通过对波数范围在1 800~1 480内的吸收带进行曲线拟合分析, 发现感染后的叶片中折叠和螺旋结构在蛋白质二级结构中的含量比例降低; 感染前期蛋白质二级结构中转角结构含量比例增大; 感染后期玉米叶片中蛋白质二级结构的自由卷曲结构含量比例急剧增大, 该结构在感染前期和健康叶片中均未出现.研究表明傅里叶变换红外光谱结合曲线拟合分析技术可用于探索玉米叶片被南方锈病侵染时所含化学物质的变化, 为玉米抗病性研究提供重要依据.
Abstract
In order to study biochemical parameters of maize leaf, which infected by maize southern rust, Fourier transform infrared spectroscopy was applied to obtain the spectra of infection and normal maize leaves. The characteristic region and fingerprint region at spectrum were selected to study the change of protein and carbohydrate in maize leaf. Compared infection with normal leaf spectrum, amide bands had diversification obviously, the change of absorption band of carbohydrate was not prominence. It showed that the protein secondary structures had changed, and the diversification of carbohydrate in infection leaf was not obviously. Curve-fitting analysis of wave number at 1 800~1 480 presented the sheet and helix in protein secondary structures was decrease in infected leaf, and the turn structure was increase at the infected initial stage. The random coil structure was increase rapidly at infected end stage, this structure was absent at infected initial stage and normal maize leaves. The results show that Fourier transform infrared spectroscopy combine with curve-fitting analysis can be used to explore the change of biochemical parameters in infection maize leaf, which provides evidence for studying the disease resistance of maize.
时有明, 李栋玉, 刘刚, 易时来. FTIR研究南方锈病侵染对玉米叶片中蛋白质和碳水化合物的影响[J]. 光子学报, 2014, 43(9): 0930003. SHI You-ming, LI Dong-yu, LIU Gang, YI Shi-lai. Influence of Protein and Carbohydrate in Maize Leaf by Infection of Maize Southern Rust Using FTIR[J]. ACTA PHOTONICA SINICA, 2014, 43(9): 0930003.