激光与光电子学进展, 2014, 51 (12): 121204, 网络出版: 2014-11-19   

绿光液晶光阀的伽马校正及空间干涉显微成像研究

Gamma Correction for Green Light Liquid Crystal Spatial Light Modulator and Spatial Light Interference Microscopy Imaging Study
作者单位
福建师范大学光电信息与工程学院, 福建 福州 350007
摘要
近年来,定量相位成像技术在生物组织及细胞成像方面受到广泛的运用。定量成像技术最初采用的光源主要为激光和超发光二极管,如今主要采用白光成像,它可以克服散斑的影响,提高轴向分辨率。介绍了低相干测量技术相关原理,利用16 阶二元相位光栅衍射的方法实现了对Holoeye Pluto 数字电寻址式液晶空间光调制器(SLM)绿光伽马校正,从而得到了相位与灰度之间的线性关系。搭建实验装置,在同一光路中完成伽马校正与定量成像。计算出奥林巴斯的20×物镜相衬环的实际大小,制作相位分别为0,π/2 ,π 和3π/2 的相位环光栅。加载相位环光栅到液晶空间光调制器上完成对散射光的相位调制。确认了绿光可运用于空间干涉显微(SLIM)调制,并初步实现了SLIM观察,对今后SLIM 研究具有一定的意义。
Abstract
In recent years,quantitative phase imaging is widely used in biological tissues and cell imaging. At first, used laser or superluminescent diode is used as the light source in quantitative phase imaging. Now it mainly adopts the white light to image, as it can overcome the influence of speckle and improve the axial resolution. In this paper, the principle of low-coherence interferometry techniques is introduced. Diffractive method with 16 order binary grating is utilized to realize the green light gamma correction of the Holoeye Pluto digital electric-addressed liquid crystal spatial light modulator, and the linear relationship between phase and gray is obtained. An experiment setup is constructed (SLM), the gamma correction and the quantitative phase imaging can be completed in the same setup. The actual size of the phase ring is calculated in the 20× objective of the Olympus, and four phase grating rings are made, their phase corresponding to 0, π/2 , π and 3π/2 . The four phase grating rings are loaded on the liquid crystal SLM to complete the phase modulation of the scattered light. It shows that the green light can be applied to spatical light interference microscopy (SLIM), and realizes the SLIM observation. It has certain significance for the SLIM study in the future.

陈大强, 郑明杰, 李志芳, 吴华钦, 王由平. 绿光液晶光阀的伽马校正及空间干涉显微成像研究[J]. 激光与光电子学进展, 2014, 51(12): 121204. Chen Daqiang, Zheng Mingjie, Li Zhifang, Wu Huaqin, Wang Youping. Gamma Correction for Green Light Liquid Crystal Spatial Light Modulator and Spatial Light Interference Microscopy Imaging Study[J]. Laser & Optoelectronics Progress, 2014, 51(12): 121204.

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