光散射学报, 2014, 26 (1): 73, 网络出版: 2014-12-08
光谱法研究吖啶橙与明胶蛋白质的相互作用
Study on the Interaction of Acridine Orange with Gelatin by Spectrometry
吖啶橙 明胶 荧光光谱 荧光猝灭 结合常数 acridine orang gelatin fluorescence spectroscopy fluorescence quenching binding constant
摘要
在pH=7.0及不同温度下, 利用荧光法、同步荧光法和紫外-可见吸收光谱研究了吖啶橙和明胶蛋白质的相互作用。荧光猝灭可分为静态猝灭和动态猝灭两种方式。通过实验可知吖啶橙与明胶属于静态荧光猝灭; 得不同温度下的反应猝灭常数KSV(288 K:7.831×103 L/mol; 303 K:6.908×103 L/mol; 318 K:5.777×103 L/mol), 且用同步荧光技术考察了吖啶橙对明胶蛋白质构象的影响。
Abstract
The interaction between acridine orange and gelation was investigated by fluorescence spectroscopy, UV-Vis spectra and synchronous fluorescence spectra. The fluorescence quenches may divide into static quenching and dynamic quenches. Fluorescence quenching of acridine orange and the gelatin belongs to static fluorescence quenching. The quenching constants (KSV) between acridine orange and gelation were (288 K:7.831×103 L/mol; 303 K:6.908×103 L/mol; 318 K:5.777×103 L/mol), respectively. The effect of acridine orange on the conformation of gelation was analyzed by synchronous fluorescence spectroscopy.
王欢, 陈佑宁. 光谱法研究吖啶橙与明胶蛋白质的相互作用[J]. 光散射学报, 2014, 26(1): 73. Wang Huan, Chen You-ning. Study on the Interaction of Acridine Orange with Gelatin by Spectrometry[J]. The Journal of Light Scattering, 2014, 26(1): 73.
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