激光治疗的研究和临床应用缺少有效反馈指导，导致治疗效果高度不稳定，针对这一问题提出激光辐照与扫频光学相干层析成像(OCT)系统整合以实现组织光热作用过程中的实时监测。利用OCT 的M 模式成像，采集激光照射过程中皮肤样品同一位置随时间变化的干涉光谱信号，测量信号深度分辨复反射率，通过其幅度和相位变化分析来探测热效应诱导组织变形过程中光散射中心位置和轴向位移的变化，并基于OCT 散射模型获取组织在激光辐照过程中光衰减系数变化信息，从而实时监测热损伤边界和损伤程度的演变。实验结果显示在一定的激光功率辐射下，随着激光作用时间的增长，组织出现热凝固坏死，热损伤边界单调下移，伴随损伤加重组织光衰减系数非线性增加。通过与光学显微、组织染色切片检测的对比分析进一步验证了该技术应用于精确监测和指导光热治疗的可行性。

Previous studies and current clinical practices have applied laser therapy without effective feedback and guidance, resulting in highly variable outcomes. A method of integrating laser therapy and sweptsource optical coherence tomography (OCT) is presented to implement real-time monitoring of tissue thermal interaction process. OCT interference spectral signals are continuously acquired in M-mode fashion without translating the skin sample, yielding depth-resolved measurement of the same axial line as a function of time during laser exposure. Thermal injury evolution is monitored quantitatively in real time through analysis of the measured complex reflectivity, in which variations in the magnitude and changes in the phase of the complex reflectivity are extracted to detect the overall aggregate changes of the scattering centers and measure the displacement in the axial direction. Meanwhile, attenuation coefficient is obtained to determine the extent of tissue thermal damage based on OCT scattering model. The experiment results indicate that thermal coagulation and necrosis occurs under irradiation of a certain laser power with the time lasting. During the process, the thermal injury boundary propagates downward monotonically and the optical attenuation coefficient nonlinearly increases. The demonstrated correspondence with microscopy and histologically determined injury depth and magnitude suggests that these techniques may enable precise monitoring and mapping of laser thermal therapy.