光谱学与光谱分析, 2015, 35 (1): 189, 网络出版: 2015-01-28  

基于金纳米粒子比色法检测全氟辛烷磺酸

Colorimetric Assay of Perfluorooctanesulfonate Based on Gold Nanoparticles
作者单位
1 发光与实时分析化学教育部重点实验室, 西南大学化学化工学院, 重庆 400715
2 重庆市纤维检验局, 重庆 401121
摘要
全氟辛烷磺酸(perfluorooctanesulfonate, PFOS)具有遗传毒性、生物蓄积性和持久性, 且难以降解, 因此对其进行分析研究具有十分重要的意义。实验发现, PFOS能使巯基乙胺包被的正电金纳米粒子发生聚集, 引起体系吸收信号及颜色改变, 据此建立了检测PFOS的紫外-可见分光光度法及比色法。线性方程为A=-0.346+0.049c, 相关系数为0.992 4, 线性范围0.8~8.0 μmol·L-1, 检出限为80 nmol·L-1。研究表明: 金纳米粒子在524 nm有特征吸收峰, 在650 nm处有较宽吸收峰, PFOS的加入会使金纳米粒子524 nm吸收峰降低, 650 nm吸收峰增强, 随着PFOS浓度增大, 体系颜色由酒红色向红紫色变化。表征了体系的扫描电镜显微成像(SEM)及紫外吸收光谱, 考察了金纳米粒子的聚集情况, 实验缓冲体系选用pH 5.0的HAc-NaAc缓冲溶液。本方法具有简单、快速等特点, 可通过肉眼观察颜色变化来实现对环境污染物PFOS的检测。本方法用于实际水样中PFOS的检测, RSD≤4.4%。
Abstract
For the property of persistent, bioaccumulation and genetic toxicity, perfluorooctanesulfonate (PFOS) is classified as a sort of persistent organic pollutants (POPs). It is significant to develop a novel assay for the determination of PFOS. In this work, we create a new colorimetric assay for PFOS in which the positively-charged gold nanoparticles (AuNPs) work as a nanoprobe. This method works on the aggregation of AuNPs induced by PFOS via electrostatic interaction. The stable monodisperse AuNPs coated by cysteamine present color of red wine and the addition of PFOS can make the monodispersed AuNPs aggregated resulting in the color change from wine red to reddish purple with a red-shift in ultraviolet-visible absorption spectrum. The experimental results show that AuNPs has a characteristic absorption peak (524 nm), as well as a wide absorption peak (650 nm) and the absorption signal intensity is proportional to the PFOS content in a range of 0.8~8.0 μmol·L-1. According to these, we developed a method based on ultraviolet-visible absorption and colorimetric to detect PFOS with the detection limit of 80 nmol·L-1. The scanning electron microscope (SEM) was investigated and the photos show that the stable AuNPs are made and the degree of AuNPs aggregation is related with PFOS concentration. The effect tests of coexisting substances in system show that common anions had less impact on the system and inorganic metal ions had some interference, which can be get rid of by cation exchange resin in real sample. This assay was applied to detect PFOS in tap water with a recovery range of 87.5%~118% and RSD≤4.4%. It is a novel application of AuNPs-based probe for PFOS detection. The proposed method has more advantages such as rapidity, low-cost and simplicity than conventional ones. In addition, it has the visual sensing function and the difference of color can be sensed by naked eyes directly, which produce ideas of real-time colorimetric strategies of nanoprobe application in environmental pollutant detection.

丛妍斌, 郑永红, 郑莉, 吴飞, 谭克俊. 基于金纳米粒子比色法检测全氟辛烷磺酸[J]. 光谱学与光谱分析, 2015, 35(1): 189. CONG Yan-bin, ZHENG Yong-hong, ZHENG Li, WU Fei, TAN Ke-jun. Colorimetric Assay of Perfluorooctanesulfonate Based on Gold Nanoparticles[J]. Spectroscopy and Spectral Analysis, 2015, 35(1): 189.

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