激发光强和激发持续时间是快速光脉冲藻类光合作用测量方法的关键实验条件.通过光脉冲激发实验, 定量分析了不同平均激发光强下还原态初级电子受体的比例和发生再氧化的初级电子受体的比例.结果表明：快速光脉冲激发的最佳平均光强为30 000 μmol quanta·m-2·s-1, 最佳激发持续时间为70 μs;30 000 μmol quanta·m-2·s-1平均激发光强能够在70 μs内还原96.08%的初级电子受体, 且仅9.81%的初级电子受体发生了再氧化.

The excitation intensity and excitation duration are the essential experimental conditions of the high-frequency flash method for the measurement of algae photosynthetic parameters. The fraction of reduced primary electron acceptors and the reoxidation extent of these reduced primary electron acceptors were analyzed under different excitation intensities and excitation durations. The analysis results indicate that 30 000 μmol quanta·m-2·s-1 is the optimal excitation intensity and 70 μs is the optimal excitation duration. Under this optimal excitation condition, 96.08% of the primary electron acceptors are reduced and only 9.81% primary electron acceptors reoxidize.