首页 > 论文 > 发光学报 > 36卷 > 3期(pp:312-316)

CdSe量子点“开关”的构建及其在药物分析中的应用

Construction of CdSe Quantum Dots “Switch” and Its Application in Pharmaceutical Analysis

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

以CdCl2·2.5H2O,Na2SeO3和NaBH4为反应物,制备巯基丁二酸稳定的CdSe量子点.在合成的量子点中加入一定浓度的铜离子,铜离子结合于量子点表面而使其荧光猝灭.在猝灭后的体系中加入青霉胺,由于青霉胺能使结合于量子点表面的铜离子释放出来而使量子点的荧光恢复.据此,建立了一种CdSe量子点“开关”测定青霉胺的新方法.在选定的实验条件下,方法的线性范围为1~72 μg/mL,检出限为0.031 μg/mL.应用于实际样品中青霉胺的测定,结果令人满意.

Abstract

CdSe quantum dots were prepared by the reaction of CdCl2·2.5H2O,Na2SeO3 and NaBH4 in water and in the presence of mercaptosuccinic acid as stabilizer.The fluorescence of quantum dots can be significantly quenched by copper ions.However,in the presence of penicillamine,copper ions were released from the quantum dots and thus restored the fluorescence of quantum dots.A new type of fluorescence turn-on assay for detection of penicillamine using quantum dots/copper ion ensemble is developed.Under the selected conditions,the response was linearly proportional to the concentration of penicillamine from 1 to 72 μg/mL,the limit of detection was 0.031 μg/mL.The developed method has been successfully applied to the detection of penicillamine in real samples with satisfactory results.

Newport宣传-MKS新实验室计划
补充资料

中图分类号:O482.31

DOI:10.3788/fgxb20153603.0312

所属栏目:材料合成及性能

基金项目:广西壮族自治区教育厅科研基金(2013YB014);广西石化资源加工及过程强化技术重点实验室开放基金(2013K007)

收稿日期:2014-11-27

修改稿日期:2015-01-06

网络出版日期:--

作者单位    点击查看

杨昆:广西大学化学化工学院 广西石化资源加工及过程强化技术重点实验室, 广西 南宁 530004
陈林情:广西大学化学化工学院 广西石化资源加工及过程强化技术重点实验室, 广西 南宁 530004
赵慧凯:广西大学化学化工学院 广西石化资源加工及过程强化技术重点实验室, 广西 南宁 530004
王益林:广西大学化学化工学院 广西石化资源加工及过程强化技术重点实验室, 广西 南宁 530004
秦泽华:广西大学 材料科学与工程学院, 广西 南宁 530004

联系人作者:杨昆(791380766@qq.com)

备注:杨昆(1990-),女,山东枣庄人,硕士研究生,2012年于中南民族大学获得学士学位,主要从事量子点荧光材料的合成与应用的研究.

【1】Saetre R,Rabenstein D L.Determination of penicillamine in blood and urine by high performance liquid chromatography[J].Anal.Chem.,1978,50(2):278-280.

【2】Amarnath K,Amarnath V,Amarnathk M,et al.A specific HPLC-UV method for the determination of cysteine and related aminothiols in biological samples[J].Talanta,2003,60(6):1229-1238.

【3】Zinellu A,Carru C,Sotiga S,et al.Plasma d-penicillamine redox state evaluation by capillary electrophoresis with laser-induced fluorescence[J].J.Chromatogr.B,2004,803(2):299-304.

【4】Yang X,Yuan H,Wang C,et al.Determination of penicillamine in pharmaceuticals and human plasma by capillary electrophoresis with in-column fiber optics light-emitting diode induced fluorescence detection[J].J. Pharm.Biomed.Anal.,2007,45(2):362-366.

【5】Shahrokhian S,Bozorgzadeh S.Electrochemical oxidation of dopamine in the presenceof sulfhydryl compounds: Application to the square-wave voltammetric detection of penicillamine and cysteine[J].Electrochim.Acta,2006,51(20):4271-4276.

【6】Torriero A A J,Piola H D,Martinez N A,et al.Enzymatic oxidation of tert-butylcatechol in the presence of sulfhydryl compounds: Application to the amperometric detection of penicillamine[J].Talanta,2007,71(3):1198-1204.

【7】He M,Zhao S L,Chen J.Enantioseparation of penicillam ine by capillary electrophoresis[J].Chin.J.Anal.Chem.(分析化学),2006,34(5):655-658 (in Chinese).

【8】Corominas B G,Pferzschner J,Icardo M C,et al.In situ generation of Co(Ⅱ) byuse of a solid-phase reactor in an reactor in an FIA assembly for the spectrophotome tric determination of penicillamine[J].J.Pharmaceut.Biomed.Anal.,2005,39(1-2):281-284.

【9】Yang J Y,Shen A Z,Du L M,et al.Determination of penicillamine using fluorescence probe of complexation between berberine and cucurbituril[J].Chin.J.Anal.Chem.(分析化学),2010,38(12):1813-1816 (in Chinese).

【10】Zhang J L,Lan C L,Li H L,et al.Spectrophotometric determination of penicillamine in penicillamine tablets[J].Phys.Testing and Chem.Anal.,Part B: Chem.Anal.(理化检验,化学分册),2014,50(1):40-42 (in Chinese).

【11】Xu Z H,Wang H W,Hou X F,et al.A novel ratiometric colorimetric and NIR fluorescent probe fordetecting Cu2+ with high selectivity and sensitivity based onrhodamine-appended cyanine[J].Sens.Actuat.B,2014,201:469-474.

【12】Yin P F,Gong H P,Li P P,et al.Fluorescence spectrometry and UV-Vis absorption spectra analysis of interaction of core-shell CdTe/CdS QDs with palmatine chloride and its applications[J].Chem.J.Chin.Univ.(高等学校化学学报),2012,33(7):1432-1437 (in Chinese).

【13】Wang Q S,Ye F Y,Fang T T,et al.Bovine serum albumin-directed synthesis of biocompatible CdSe quantum dots and bacteria labeling[J].J.Colloid Interf.Sci.,2011,355(1):9-14.

【14】Fan J,Song J,Bi L R,et al.Sandwich immunosensing method for measuring cardiac troponin I with quantum dots-coup led antibody against troponin I[J].Chem.J.Chin.Univ.(高等学校化学学报),2009,30(11):2180-2183 (in Chinese).

【15】Zhang K,Mei Q S,Guan G J,et al.Ligand replacement-induced fluorescence switch of quantum dots for ultrasensitive detection of organophosphorothioate pesticides[J].Anal.Chem.,2010,82(22):9579-9586.

【16】Hong J Q,Pei D J,Guo X Q.Quantum dot-Eu3+ conjugate as a luminescence turn-on sensor for ultrasensitive detection of nucleoside triphosphates[J].Talanta,2012,99:939-943.

【17】Li S H,Tao H L,Xu M Z,et al.A new type CdTe quantum dots “switch” and its application in determination of norfloxacin[J].Chin.J.Anal.Chem.(分析化学),2012,40(9):1450-1453 (in Chinese).

【18】Gao M X,Xu J L,Li Y F,et al.A rapid and sensitive spectrofluorometric method for 6-mercaptopurne using CdTe quantum dots[J].Anal.Methods,2013,5:673-677.

【19】Ruedas-Rama M J,Hall E A H.Azamacrocycle activated quantum dot for zinc ion detection[J].Anal.Chem.,2008,80(21):8260-8268.

【20】Varela A T,Stevenson E I,Gasion J A G.Selective turn-on fluorescence detection of cyanide in water using hydrophobic CdSe quantum dots[J].Chem.Commun.,2008,44(17):1998-2000.

【21】Wang Y L,Yang K,Pan H Q,et al.Preparation and characterization of high quality CdSe quantum dots in aqueous solution[J].Chem.J.Chin.Univ.(高等学校化学学报),2012,33(12):2604-2608 (in Chinese).

引用该论文

YANG Kun,CHEN Lin-qing,ZHAO Hui-kai,WANG Yi-lin,QIN Ze-hua. Construction of CdSe Quantum Dots “Switch” and Its Application in Pharmaceutical Analysis[J]. Chinese Journal of Luminescence, 2015, 36(3): 312-316

杨昆,陈林情,赵慧凯,王益林,秦泽华. CdSe量子点“开关”的构建及其在药物分析中的应用[J]. 发光学报, 2015, 36(3): 312-316

被引情况

【1】李正顺,岳圆圆,张艳霞,王岩,王雷,王海宇. 丁胺包裹的CdSe量子点敏化的TiO2纳米晶薄膜电子转移机制. 中国光学, 2015, 8(3): 428-438

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF