针对近红外光谱可对活体组织进行无损伤穿孔,并解决在穿孔中需要重新对焦的问题,通过Zemax 软件对7 片式前置光阑显微物镜结构在0.48～1.48 ?m 波长范围内进行了宽光谱消色差的优化设计.设计实现了像距为无限远的多光谱共焦成像,其放大倍率为40,数值孔径0.5,最大焦面漂移量仅为27 ?m,可见光波段在空间频率为350 lp/mm 时,0.7 视场的MTF＞0.5,具有较高的分辨力.并且在波长1480 nm 处,MTF 值接近衍射极限,具有较高的能量,满足细胞穿孔的要求.文中还给出了实验结果,并预测其应用前景.

Since living tissue can be perforated without injury bynear infrared spectrum,and in order to solve the problem of refocusing,the 7 pieces of front aperture microscope structure is designed and optimized in the 0.48-1.48 ?m waveband by using Zemax software.It realizes apochromatism and multi-spectral confocal imaging with the image distance of infinity,and the maximum focal shift is only 27 ?m.At the same time,the optical magnification reaches 40 and number aperture is 0.5.The Optimal Structure Designing shows that the system has high resolution,the MTF of visible wavelengths beyond 0.5 at a spatial frequency of 350 lp/mm in 0.7 field.In addition,the MTF of 1480 nm wavelength is close to the diffraction limit and the energy is high,which caters for the requirements of cell perforation.In the meantime,the experimental results are drawn out and we have made predictions about its prospects.