光学仪器, 2015, 37 (2): 100, 网络出版: 2015-05-20
紫外可见分光光度计线性度测试方法
Linear test method of ultraviolet-visible spectrophotometer
摘要
为定性测试紫外可见(UV-VIS)分光光度计的线性度,以水质标准液标定法为基础,用一台紫外可见分光光度计在特定波长处测得一种食物染料的吸光度,再用基本的数学方法计算出各个测试点的体积分数,进而获得体积分数和吸光度的线性关系。实验表明,该方法可以方便地测试出仪器线性度,计算方法正确且简单易懂。
Abstract
In order to qualitatively test linearity of ultraviolet-visible (UV-VIS) spectrophotometer, we test a food-dye absorbance at a certain wavelength using a UV-VIS spectrophotometer based on the water quality standard liquid calibration method, and then use a basic mathematical method to calculate volume concentration at each test point. We get a linear relationship of volume concentration and absorbance. Experiments show that this method is easy and convenient to measure the linearity of the instrument and the calculation method is accurate and easy to understand.
刘明兰, 孙玉国, 朱轩轩. 紫外可见分光光度计线性度测试方法[J]. 光学仪器, 2015, 37(2): 100. LIU Minglan, SUN Yuguo, ZHU Xuanxuan. Linear test method of ultraviolet-visible spectrophotometer[J]. Optical Instruments, 2015, 37(2): 100.