光子学报, 2015, 44 (5): 0517003, 网络出版: 2015-05-26  

极低频脉冲电场对渗透胁迫下玉米幼苗根系细胞延迟光子辐射的影响

Effect of Extremely Low Frequency Pulsed Electric Field on Delayed Biophoton Emission of Cells in Maize Seedling Root under Osmotic Stress
作者单位
西安理工大学 应用物理系, 西安 710048
摘要
为了揭示极低频脉冲电场对作物抗旱性的影响及其机理, 采用渗透势为-0.1 MPa的PEG-6000溶液模拟干旱条件, 研究脉冲频率为1 Hz 、场强为200 kV/m、脉宽为80 ms的极低频脉冲电场对干旱胁迫下玉米幼苗根系延迟光子辐射动力学参数的影响.结果表明, 在干旱胁迫下, 玉米幼苗根系细胞延迟光子辐射动力学参数的初始光子数I0、相干时间τ和衰减参数β均随着胁迫时间的延长呈现出波动性的下降趋势, 经过极低频脉冲电场处理的玉米幼苗根系细胞的初始光子数I0、相干时间τ和衰减参数β的变化趋势与之类似, 但是, 各动力学参数的数值均明显高于对照.延迟发光动力学参数的变化暗示极低频脉冲电场处理提高了渗透胁迫下玉米幼苗根系细胞的代谢水平和组织序性, 增强了细胞内功能分子之间的相互作用.延迟光子辐射的分析结果为在活体细胞层面阐明极低频脉冲电场对作物抗旱性的影响及其机理提供了参考.
Abstract
Extremely low frequency pulsed electric field could arouse a comparatively intense reaction on physiological characteristics of plant cells, so the effect of the extremely low frequency pulsed electric field on crops drought resistance is very worthy to be studied. The delayed biophoton emission of cells, as a kind of life information, can reflect the changes of cellular physiological state. In order to study the influence of the extremely low frequency pulsed electric field on drought resistance of plants seedlings and its mechanism, the PEG-6000 solution with the osmotic potential of -0.1 MPa was used to simulate drought conditions, the germinating maize was treated by the extremely low frequency pulsed electric field at 100 kV/m, frequency 1 Hz and pulse width 80 ms, the changes of the delayed biophoton emission from maize seedling roots under osmotic stress were discussed. The results show that the changes of the kinetic parameters about delayed biophoton emission such as the initial photon number I0, coherence time τ and decay factor β decrease volatility. The changes of the kinetic parameters about delayed biophoton emission of maize seedling root treated by extremely low frequency pulsed electric field are the same, but, the value of these parameter increase significantly in comparison with the control. The analysis indicates that the extremely low frequency pulsed electric field treatment can raise the level of the metabolic level of cell and improve tissue order, the interactions between functional molecules in cells is enhanced. The results of this paper provide a reference for explaining clearly the effect of extremely low frequency pulsed electric field on drought resistance of plants seedlings and its mechanism.

贺瑞瑞, 习岗, 刘锴, 赵燕燕. 极低频脉冲电场对渗透胁迫下玉米幼苗根系细胞延迟光子辐射的影响[J]. 光子学报, 2015, 44(5): 0517003. HE Rui-rui, XI Gang, LIU Kai, ZHAO Yan-yan. Effect of Extremely Low Frequency Pulsed Electric Field on Delayed Biophoton Emission of Cells in Maize Seedling Root under Osmotic Stress[J]. ACTA PHOTONICA SINICA, 2015, 44(5): 0517003.

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