由于光子晶体滤波器带宽窄和高灵敏度的特点,检测抗体含量极限可以达到ng·mL-1.可以应用于生物传感器监测生物大分子内部反应过程.一维光子晶体滤波器作为生物传感器换能元件将生物信息转化为可检测的光电信号信息,主要反应在光谱仪测得共振波峰所在波长的变化.精确测试时,检测传感系统的稳定性是首要考虑的关键因素.稳定性决定实验数据的有效性.该工作对所制备的基于导模共振效应的一维光子晶体滤波器结构进行形貌和光谱测试,介绍了实验室所搭建的实时检测传感系统.由于系统集成度、耦合损耗等因素的影响,光谱信息出现一定的噪声信号.因此,重点提出利用Lab VIEW编程实时监控光子晶体滤波器共振波峰值随时间的变化,由光谱变化情况来反应本套检测系统的稳定性和测试数据的有效性.同时这种实时监控程序也可用来监控其他基于导模共振生物传感系统的稳定度.该检测系统由于震动,光源抖动等问题产生的共振峰值漂移为0.25 nm,通过与模拟计算结果比较可以判定系统稳定性可以达到试剂检测的要求.

The detection limit of antibody content has reached level of nanograms per milliliter due to high sensitivity and extremely narrow band of photonic crystal(PC) filter.The PC filter based on guided-mode resonance(GMR) effect can also be applied to detecting the molecular interactions.As the transducing element,one-dimensional PC filters transform biological information to photoelectric signal on optical spectrum analyzer(OSA).The main sensing performance is the change of peak-wavelength of PC filter.The sensing system using PC filter is restricted to the system stability which determines the effectiveness of detecting data.So in this paper,a detecting system we designed is briefly addressed.The morphology and the spectrum of PC filter we fabricated are tested.Considering the coupling light loss and integration of the system,noise signal in spectrum is going to affect the detecting results.To monitor the influence,realization of real-time monitoring the changes of the peak wavelength of PC filter is mainly illustrated.The monitoring is realized by transferring detecting data to computer in time and the results can represent the stability of the system.The program is compiled by Lab VIEW.In our experiment,the shift of 0.25 nm of the peak wavelength caused by vibration of platform or unsteadiness of light source is within the sensitivity of the PC filter obtained by simulation,so we proposed this system we mentioned can be used in sensing most kind of bulk reagents.