发光学报, 2015, 36 (6): 705, 网络出版: 2015-06-25
盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究
Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy
荧光猝灭法 同步荧光法 盐酸吡格列酮 牛血清白蛋白 反应机理 fluorescence spectroscopy synchronous fluorescence spectroscopy pioglitazone hydrochloride bovine serum albumin reaction mechanism
摘要
利用荧光猝灭法和同步荧光法进行了盐酸吡格列酮(PGH)与牛血清白蛋白(BSA)之间反应机理的比较研究.结果表明:两种方法在研究PGH-BSA体系的猝灭类型、结合常数、结合位置、作用力类型、协同性及能量转移距离等方面,得到了相同的结果,表明同步荧光光谱法可以用于药物与蛋白反应机理的研究.
Abstract
The reaction mechanism between pioglitazone hydrochloride (PGH) and bovine serum albumin (BSA) was studied by fluorescence quenching method and synchronous fluorescence method under different temperatures.The results show that the conclusions of two methods are identical on the quenching mechanism,type of interaction force,binding constants,binding location,synergistic effect,energy-transfer parameters of pioglitazone hydrochloride with bovine serum albumin,etc.These indicate that the synchronous fluorescence spectroscopy can be used to replace the traditional fluorescence spectroscopy to study the reaction mechanism of drugs with protein.
李改霞, 刘保生, 李志云, 付家赫, 王天成. 盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究[J]. 发光学报, 2015, 36(6): 705. LI Gai-xia, LIU Bao-sheng, LI Zhi-yun, FU Jia-he, WANG Tian-cheng. Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2015, 36(6): 705.