850nm微激光已被发现能够减少黑色素细胞内酪氨酸酶的表达,进而抑制黑色素的合成.在此基础上本文选用C57BL/6J小鼠为模型,采用拉曼光谱法和荧光光谱法初步研究了850 nm微激光的在体美白效应.黑色素含量丰富时,小鼠皮肤拉曼谱线中代表黑色素的信号(1 405 cm-1和1 609 cm-1)变得异常明显;而经过微激光的照射后这些拉曼信号明显减弱.在NIR光的激发下黑色素也能发出强烈的荧光,这种荧光信号也会因微激光的照射而减弱.通过计算相对的拉曼信号强度及荧光信号强度对850 nm微激光的美白效应进行了定量分析.最后,采用常规的组织学检测(H&E染色和Fontana-Masson染色)验证了光谱学检测手段的有效性.因此,光谱学技术可以作为一种简单有效的方法用于黑色素相关生理事件的实时检测.

It has been found that the synthesis of melanin in melanophores was suppressed after 850 nm micro-laser irradiation,due to the reduced expression of tyrosinase.Herein,a preliminary study of the in vivo whitening effect of 850 nm micro-laser on C57BL/6J mice was carried out using Raman spectroscopy and fluorescence spectroscopy.The characteristic peaks of melanin(1 405 cm-1 and 1 609 cm-1)became obvious when the skin tissue was abundant of melanin.The Raman signals of melanin reduced significantly after 850 nm micro-laser irradiation.Melanin also emitted strong fluorescence under NIR excitation,which also reduced after micro-laser irradiation.The relative Raman intensities and fluorescence intensities were then calculated to quantitatively analyze the whitening effect of 850 nm micro-laser.Finally,the availability of spectroscopic detection means was verified by routine histological examinations(H&E and Fontana-Masson staining).As a result,spectroscopic technology was a simple and effective means for real-time detection of melanin related physiological events.