激光生物学报, 2015, 24 (1): 68, 网络出版: 2015-06-26
胃癌血清的表面增强拉曼光谱研究
The Study of Gastric Cancer Serum on Surface Enhanced Raman Spectroscopy Technology
表面增强拉曼光谱(SERS) 胃癌 血清 金溶胶 Surface Enhanced Raman Spectroscopy(SERS) gastric carcinoma serum gold nanoparticle
摘要
表面增强拉曼光谱技术在癌症诊断领域已经有了广泛的研究和应用,本文利用金纳米溶胶为增强基底,采用便携式近红外拉曼光谱系统,对89例胃癌患者及正常人血清样本进行了SERS光谱探测.结果表明,血清的特征峰主要归属于氨基酸,其次是核酸、糖类及脂类.相比于正常人血清SERS光谱,胃癌血清中归属于核酸的特征峰强度都较高,大部分归属于蛋白质的特征峰强度较低,与医学研究结论相符.说明SERS技术可以有效地反映胃癌患者和正常人血清的差异,为后期SERS技术快速诊断胃癌提供了实验基础.
Abstract
Surface-enhanced Raman spectroscopy has been extensively studied and applied in cancer diagnosis field.In this paper,gold colloid was prepared as SERS substrate and its enhanced factor was calculated using crystal violet molecules as probes,and the SERS spectra of serum of gastric cancer patients and healthy people were detected.The enhancement effect of SERS spectra of serum using gold colloid as SERS substrate was significant comparing with Raman spectra of serum.The Raman peaks were mainly assigned to amino acid,nucleic acid,and carbohydrate etc.Comparing with the healthy people,the Raman intensities of nucleic acids of gastric cancer patients were higher and that of proteins were lower,which coincided with the conclusion of medical researches.It indicates that SERS is suitable for distinguishing gastric carcinoma and provides a promising approach for gastric cancer diagnosis.
孟辰, 周涵婧, 史晓凤, 毛伟征, 马君. 胃癌血清的表面增强拉曼光谱研究[J]. 激光生物学报, 2015, 24(1): 68. MENG Chen, ZHOU Hanjing, SHI Xiaofeng, MAO Weizheng, MA Jun. The Study of Gastric Cancer Serum on Surface Enhanced Raman Spectroscopy Technology[J]. Acta Laser Biology Sinica, 2015, 24(1): 68.