应用激光, 2015, 35 (3): 330, 网络出版: 2015-07-10  

人成骨细胞在宽带激光熔覆Nd2O3活性涂层表面的成骨性能

0steogenic properties of Human Osteoblasts in the Rare Earths Bioceramic Coating surface Produced by Wide-band Laser Cladding
作者单位
贵州交通职业技术学院, 贵州 贵阳 550008
摘要
采用宽带激光熔覆技术,梯度设计的思想,添加不同含量稀土氧化物Nd2O3来提高复合涂层生物活性的方法,在TC4钛合金表面制备了含HA+β-TCP的稀土梯度生物活性陶瓷涂层.使用MG63人成骨细胞与Nd2O3活性梯度涂层体外共培养,用MTT法测定成骨细胞碱性磷酸酶含量和通过SEM观察MG63细胞在活性涂层表面上的伪足生长情况.结果表明:稀土梯度生物活性陶瓷涂层碱性磷酸酶(ALP)的表达量均高于TC4合金和未加入Nd2O3的陶瓷涂层,成骨活性较好;成骨细胞向骨细胞分化能力是逐渐增强的;成骨活性与不同含量的Nd2O3合成的HA+β-TCP的数量密切相关,当Nd2O3的添加量为0.6wt.%时,具有最佳的成骨性能;生物活性复合涂层不仅能引起细胞的粘附生长,更重要的是能够促进细胞的定向分化,且统计学分析表明ALP含量具有明显的显著性.稀土活性梯度陶瓷涂层材料表面细胞伪足生长更旺盛,具有更好的生物相容性.
Abstract
In this paper,as to problem on bioceramic coating at present,a kind of gradient bioceramic coating is designed,the rare earths active gradient bioceramic coating with HA and β-TCP on TC4 titanium alloy was prepared by using wide-band laser cladding technique.The method was to culture osteoblast on gradient bioceramic coating in vitro.Results show that alkaline phosphatase expression of the rare earths active gradient bioceramic coating was higher than that of TC4 alloy and without Nd2O3 bioceramic coating,the osteogenic activity is better; The rare earths active gradient bioceramic coating can not only cause the cell adhesion and growth,more important is to be able to promote the differentiation of cells.pseudopods of surface cell of the rare earths active gradient bioceramic coating material grow more exuberant,thus with better biocompatibility.

汪震, 田兴强, 李宾. 人成骨细胞在宽带激光熔覆Nd2O3活性涂层表面的成骨性能[J]. 应用激光, 2015, 35(3): 330. Wang Zhen, Tian XingQiang, Li Bin. 0steogenic properties of Human Osteoblasts in the Rare Earths Bioceramic Coating surface Produced by Wide-band Laser Cladding[J]. APPLIED LASER, 2015, 35(3): 330.

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