采用2.71 GHz脉冲微波(脉冲场强为48 kV/m,脉宽为750 ns,脉冲个数分别为200个×1次、200个×10次、200个×20次)辐照SD雄性大鼠和孕鼠,利用流式细胞术、计算机辅助精子分析系统及水迷宫实验分别检测了精子发生过程中生精细胞DNA损伤情况、附睾内精子活力参数变化及子代学习记忆功能.结果表明:2.71 GHz脉冲微波单次辐照会对雄性大鼠的生精过程产生一定的影响,但对附睾内精子质量无明显影响,而多次辐照对精子质量的影响具有累积效应;亲代大鼠在受照后,其子代的生长在短期会受阻,且学习、记忆能力也会受到一定的影响.

The effect of pulse microwave irradiation on the reproductive system of animal and human as well as their offspring is a critical issue that causes interests.In present work,the parental rats were exposed to 2.71 GHz pulses microwave(field strength 48 kV/m,pulse width 750 ns,pulse number 200×1,200×10,200×20,respectively),and the DNA damage during the spermatogenesis process,the sperm motility,the growth of the baby offspring,and the learning and memory ability of the adult offspring were studied by means of DNA staining,TUNEL staining,Elisa assay,sperm motility CASA detection,and water maze experiment.The experimental results show that the single irradiation on male rats affects their early spermatogenesis process rather than the sperm motility,while multi-times of irradiation decrease the sperm motility due to the accumulative effect.The growth of baby offspring is temporarily blocked,and the learning and memory ability also trends to be blocked when the parent rats receive the irradiation.