激光生物学报, 2014, 23 (2): 165, 网络出版: 2015-07-20
福建省马铃薯晚疫病菌线粒体DNA单倍型分析
Identity of the mtDNA Haplotype(s) of Phytophthora Infestans in Fujian Province
摘要
为明确福建省马铃薯晚疫病菌线粒体DNA单倍型组成和分布情况, 采用PCR-RFLP方法分析了2010-2012年从福建省7个地区(福州, 长乐, 漳州, 青口, 龙海, 霞浦, 龙岩)分离的526个马铃薯晚疫病菌株的线粒体DNA单倍型及频率分布。结果表明Ⅰa型为主要单倍型, 占72.8%, 其次是Ⅱb型, 占26.42%, 最少的Ⅱa型, 占0.76%, 没有检测到Ⅰb型。与文献报道历史材料相比较, 说明福建省马铃薯晚疫病菌线粒体DNA单倍型的组成发生较大变化, 而且福建省马铃薯晚疫病菌线粒体单倍型组成比云南、四川和贵州等地区更为复杂。
Abstract
To detect the distribution of mitochondrial DNA haplotypes of Phytophthora infestans in Fujian province, 526 isolates of P. infestans collected from seven different locus in 2010 to 2012 were detected by PCR-RFLP. The most important mitochondrial DNA haplotypes was Ⅰa strains, accounting for 72.8%, and the second was Ⅱb, accounting for 26.42%, the rest 0.76% belong to theⅡa. Ⅰb strain was not detected. Compared with literatures, mitochondrial haplotype composition of P. infestans in Fujian province changed greatly, and was more complex than in other regions, such as Yunnan, Guizhou and Sichuan province et al.
韩丽丽, 杨策, 潘贤, 詹家绥, 祝雯. 福建省马铃薯晚疫病菌线粒体DNA单倍型分析[J]. 激光生物学报, 2014, 23(2): 165. HAN Lili, YANG Ce, PAN Xian, ZHAN Jiasui, ZHU Wen. Identity of the mtDNA Haplotype(s) of Phytophthora Infestans in Fujian Province[J]. Acta Laser Biology Sinica, 2014, 23(2): 165.