激光生物学报, 2013, 22 (3): 201, 网络出版: 2015-07-24
重离子辐照酿酒酵母DNA损伤修复途径研究进展
The Research Progress of DNA Damages Repair Pathways Induced by Heavy Ion Irradiation in Saccharomyces cerevisiaes
摘要
重离子辐照通过直接和间接作用导致生物体DNA产生损伤, 包括DNA的链断裂、碱基的插入或丢失以及氧化损伤等。DNA损伤直接影响复制、转录和蛋白质合成, 同时还是突变的重要原因, 因此, DNA损伤修复系统尤为重要。在酿酒酵母中, 这些损伤主要是通过同源重组修复(homologous recombination repair, HRR)、碱基错配修复(mismatch repair, MMR)和碱基切除修复(base excision repair, BER)等途径来修复的。作为真核生物研究的模式生物, 对于酿酒酵母DNA损伤修复的HRR、MMR和BER途径研究颇多, 也不断有一些新的成果出现, 特别是对于相关途径的完善和相关蛋白的深化更是研究热点, 在此对近年来有关重离子辐照酿酒酵母DNA损伤修复途径方面的研究做一综述。
Abstract
The DNA of organism can be damaged by the direct and indirect effect of heavy ion irradiation included the chain interruption of DNA, the insertion and missing of base and oxidative damage. The lesions of DNA are detrimental to organism, since they will impact replication, transcription and protein synthesis immediately. At the same time, they also are the significant cause leading to mutation. Therefore, the system of DNA damage repair is distinctly important to organism. In Saccharomyces cerevisiaes, which is used as model organism for the research eukaryotes, these damage are repaired by the pathways of HHR, MMR and BER which have been studied broadly and emerged numerous achievement continually. In particular, both relevant pathways and protein are always a hot research area. In this paper, the recent findings on the approach of DNA damages repair in S.cerevisiae are reviewed.
曹国珍, 陆栋, 张苗苗, 王菊芳, 马良, 李欣, 李文建. 重离子辐照酿酒酵母DNA损伤修复途径研究进展[J]. 激光生物学报, 2013, 22(3): 201. CAO Guozhen, LU Dong, ZHANG Miaomiao, WANG Jufang, MA Liang, LI Xin, LI Wenjian. The Research Progress of DNA Damages Repair Pathways Induced by Heavy Ion Irradiation in Saccharomyces cerevisiaes[J]. Acta Laser Biology Sinica, 2013, 22(3): 201.