发光学报, 2015, 36 (7): 854, 网络出版: 2015-08-25
化学发光检测光子计数的统计特性
Photon Counting Statistical Analysis for Chemiluminescence Detection
摘要
对于增强化学发光酶联免疫分析而言, 检测结果、计数效率、信噪比等重要参数不仅与光子计数装置有关, 而且与光学发光体系的光子脉冲发射统计过程相关。本文实验测量了增强鲁米诺化学发光体系的光子脉冲的统计特性, 并根据其脉冲发射时间统计特性对化学发光光子计数检测装置的设计进行了理论分析。研究结果表明, 化学发光的光子脉冲发射间隔呈Weibull分布。
Abstract
In enhanced chemiluminescence enzyme-linked immunoassay, major parameters such as detection result, efficiency of counting and S/N ratio, are related with not only photon counting devices but also statistics process of photon pulse emission in optical luminescence system. In this article, we measured the statistics characteristics of photon pulse in enhanced luminol chemiluminescence system and theoretically analyzed the design of photon counting detection device of chemiluminescence system by its statistics characteristics of its pulse emission time. The research suggests that the photon emission pulse intervals of chemiluminescence subject to Weibull distribution.
陈文洋, 邹明强, 刘峰, 李锦丰. 化学发光检测光子计数的统计特性[J]. 发光学报, 2015, 36(7): 854. CHEN Wen-yang, ZOU Ming-qiang, LIU Feng, LI Jin-feng. Photon Counting Statistical Analysis for Chemiluminescence Detection[J]. Chinese Journal of Luminescence, 2015, 36(7): 854.