太赫兹波（THz）是一种介于微波和红外线波之间的电磁波。由于生物体对THz波的独特响应性，太赫兹波在生物医学领域的应用研究特别是其与生物组织的相互作用成为了研究热点。该研究旨在探索太赫兹波能否激发光敏剂产生光敏效应。采用纳焦级宽谱（1~3 THz）的脉冲太赫兹光源对光敏剂（PS）血卟啉单甲醚（HMME）照射30 min，用DPBF作为单态氧的捕获剂检测单态氧产率。采用相同的太赫兹光源照射常规培养的HepG2细胞，光学显微镜下观察细胞形态，MTT法检测细胞活性。PS+THz组单态氧产率显著高于单纯太赫兹波组（21.04% vs. 2.39%）；PS+THz组HepG2细胞形态较对照组略圆，细胞有收缩趋势；细胞活性检测结果显示，太赫兹波照射后HMME孵育的HepG2细胞的活性降低至81.13%（THz组为99.21%）。实验结果表明宽谱1~3 THz纳焦级太赫兹波可激发光敏剂HMME，激发效率约为20%。

Terahertz (THz) radiation lies between the infrared and microwave regions of the electromagnetic spectrum. Because of organisms’ unique response to the THz wave, THz wave applications in biomedical research, especially in its interactions with biological tissues become a hot spot. Whether the THz wave has the photodynamic effect by exciting the photosensitizer is explored in the study. The wide spectrum (1-3 THz) of pulse terahertz is used as a light source to excite the photosensitizer-hematoporphyrin monomethyl ether (HMME) for 30 min, and then DPBF is used as singlet oxygen scavenger to test the yield of singlet oxygen. HepG2 cells in conventional culture are illuminated with the same dose of terahertz wave, the cell morphology is observed by an optical microscope, the cell activity is determined by MTT method. The singlet oxygen production in PS+THz group is significantly higher than that of pure THz wave irradiation (21.04% vs. 2.39%). Compared to control cells, HepG2 cells in PS+THz group are slightly rounded and have a tendency to shrink. The activity of HepG2 cells incubated in HMME (HMME+THz group) is reduced to 81.13% after irradiation by THz wave (99.21% in THz group). The experimental results show that the THz wave of wide spectrum (1-3 THz) and nanojoule energy can excite the photosensitizer of HMME with excitation efficiency of about 20%.