激光生物学报, 2010, 19 (4): 542, 网络出版: 2015-10-08
曲酸高产菌株的诱变选育
Mutagenesis of Strain with High Yield of Kojic Acid
摘要
以紫外线和氮离子注入作为诱变手段对曲酸产生菌黄曲霉CICC 2242进行诱变处理, 筛选出一株高产菌株N83。曲酸产量由初始的12.4 g/L提高到19.4 g/L, 提高了56.5 %; 遗传稳定性实验结果表明, N83遗传性状稳定良好。该结果表明氮离子注入诱变是获得高产曲酸的有效途径。
Abstract
The kojic acid-producing strain Aspergillus flavus CICC 2 242 was mutagenized using UV radiation and N+ implantation, and its mutant strain A.flavus N83 was obtained. The mutant strain produced kojic acid at 19.4 g/L, which was 56.5 % higher than that with A. flavus CICC 2 242 at 12.4 g/L. Moreover, A. flavus N83 showed good genetic stability. The results showed that N+ implantation was an effective method to generate better kojic acid producer.
赵鑫, 赵金, 吴思国, 胡翠敏, 赵宗保. 曲酸高产菌株的诱变选育[J]. 激光生物学报, 2010, 19(4): 542. ZHAO Xin, ZHAO Jin, WU Si-guo, HU Cui-min, ZHAO Zong-bao. Mutagenesis of Strain with High Yield of Kojic Acid[J]. Acta Laser Biology Sinica, 2010, 19(4): 542.