从农药厂排污沟污泥中分离到一株能降解毒死蜱的新菌株, 命名为R17, 经生理生化和16S rDNA 序列同源性分析, 鉴定为Sphingopyxis terrae。R17可以利用毒死蜱作为唯一碳源生长, 该菌株的最适生长温度为35 ℃、最适pH为7~8, 在此条件下培养28 h后, 菌落浓度达9.18×108 cfu/mL。研究了该菌株在不同时间, 对不同浓度毒死蜱的降解特性, 结果表明, 当接菌量为9.18×107 cfu/mL时, 在1 d、2 d、3 d和4 d内对10 mg/L的毒死蜱降解率分别达到18.59 %、31.23 %、36.55 %和47.69 %以上; 在2 d内对1 mg/L、5 mg/L和10 mg/L的毒死蜱降解率分别达到50.21 %、43.46 %和31.23 %。

One bacterial strain, named R17, was isolated from the sludge polluted with chlorpyrifos which had the capability to utilize chlorpyrifos as the sole carbon and energy sources. Strain R17 was identified preliminarily as Sphingopyxis terrae based on its physiological and biochemical characters and its 16S rDNA homologue sequence analysis. Under the optimal growth condition, 35 ℃ and pH 7~8. Strain R17 could reach 9.18×108 cfu/mL after 28 h. The chlorpyrifos degradation characteristics of strain R17 were studied with different times and different initial chlorpyrifos concentrations. The results showed that with the inoculation of 9.18×107 cfu/mL, the degradation rate of chlorpyrifos by strain R17 were 18.59 %, 31.23 %, 36.55 % and 47.69 % in 1 d, 2 d, 3 d and 4 d respectively. The degradation rate of R17 with initial concentration of 1 mg/L, 5 mg/L, 10 mg/L chlorpyrifos reached 50.21 %, 43.46 % and 31.23 % after 2 d, respectively.