激光生物学报, 2012, 21 (6): 486, 网络出版: 2015-10-08  

镉诱导拟南芥细胞程序性死亡过程中NO产生和亚细胞定位观察

Identification and Detection of Subcellular Localization of NO in Cadmium-induced Arabidopsis thaliana Programmed Cell Death
作者单位
华南师范大学生物光子学研究院激光生命科学研究所、暨激光生命科学教育部重点实验室, 广东 广州 510631
摘要
镉是一种高度有毒的重金属, 能够抑制植物生长, 甚至导致死亡, 但是其诱导细胞程序性死亡(PCD)的分子机制仍然不是很清楚。本文利用荧光探针分子成像和激光共聚焦扫描显微技术, 以拟南芥叶肉细胞原生质体为材料, 观察100 μmol/L CdCl2诱导PCD过程中一氧化氮(NO)的产生和亚细胞定位。结果表明高浓度CdCl2能够明显降低细胞活力, 100 μmol/L CdCl2能够诱导大量NO产生, 在处理12 h后NO产生达到峰值。亚细胞定位观察发现NO荧光首先和线粒体有共定位。随着处理时间的延长在叶绿体和胞质也观察到NO荧光。
Abstract
Cadmium (Cd2+), a non-essential element, exhibits high levels of toxicity. Cd2+ exposure could inhibit plant growth and even result in cell death. Yet the mechanisms of Cadmium-induced programmed cell death (PCD) remain largely unknown. Using fluorescent probes molecular imaging and laser confocal scanning microscopy, the temporal and spatial characteristics of nitric oxide (NO) production during Cd2+ -induced PCD in Arabidopsis protoplasts were investigated in vivo and in real-time. Results demonstrated that high doses of Cd2+ strongly decreased cell viability and induced large amount of NO. After 12 h treatment with 100 μmol/L Cd2+ the NO content reached a maximum. NO was first generated in the mitochondrial regions of protoplasts and subsequently in chloroplasts and even whole cell as the treating time went on.

叶芸, 李哲, 邢达. 镉诱导拟南芥细胞程序性死亡过程中NO产生和亚细胞定位观察[J]. 激光生物学报, 2012, 21(6): 486. YE Yun, LI Zhe, XING Da. Identification and Detection of Subcellular Localization of NO in Cadmium-induced Arabidopsis thaliana Programmed Cell Death[J]. Acta Laser Biology Sinica, 2012, 21(6): 486.

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