发光学报, 2015, 36 (9): 989, 网络出版: 2015-10-22  

叶酸修饰的AgInS2量子点在体外癌细胞成像上的应用

Folic Acid-conjugated AgInS2 Quantum Dots for in vitro Cancer Cell Imaging
作者单位
1 长春理工大学理学院 国际纳米光子学与生物光子学联合研究中心, 吉林 长春130022
2 南洋理工大学 电气与电子工程学院, 新加坡
摘要
成功制备出高品质的三元AgInS2量子点。通过配体交换法将油溶性AgInS2量子点转为水溶性量子点, 通过dBSA修饰水溶性量子点形成配位体壳, 使量子点具有更好的稳定性(4周)。从透射电子显微镜(TEM)观察到dBSA修饰后的量子点的粒径增加, 分散性较好, 并且在可见光区域有明显的光致发光。用叶酸对dBSA-MPA量子点进行修饰, 并通过傅立叶变换红外光谱进行了验证。将得到的FA-dBSA-MPA纳米复合材料应用于能与叶酸受体特异性结合的乳腺癌细胞中, 并在荧光倒置显微镜中检测到量子点成功对乳腺癌细胞进行了标记。与dBSA-MPA量子点相比, 表面被叶酸修饰后的量子点与癌细胞的结合效率显著提高。
Abstract
AgInS2 quantum dots (QDs) were synthesized using hot colloidal method and transferred into water via a ligand exchange route. To enhance the stability of these QDs, dBSA was coated on the aqueous QDs as a ligand shell. After dBSA modification, a size increase was observed from the transmission electron microscopy (TEM) results. The prepared dBSA-MPA QDs exhibited good monodispersity, enhanced stability (stable for 4 weeks) and bright photoluminescence. Then the QDs were functionalized with folic acid (FA) and the successful conjugation was confirmed by the Fourier transform infrared (FT-IR) spectroscopy. The resulting FA-dBSA-MPA QDs nanocomposites were tested in breast cancer cells (MCF-7 cells) with elevated folate receptor expression. Compared to the unconjugated dBSA-MPA QDs, the presence of FA on the surface of QDs significantly improved the uptake rate of the nanoparticles by the cancer cells.

卜承飞, 刘丽炜, 王倩, 张卜天, 胡思怡, 任玉, 朱泠西. 叶酸修饰的AgInS2量子点在体外癌细胞成像上的应用[J]. 发光学报, 2015, 36(9): 989. BU Cheng-fei, LIU Li-wei, WANG Qian, ZHANG Bu-tian, HU Si-yi, REN Yu, ZHU Ling-xi. Folic Acid-conjugated AgInS2 Quantum Dots for in vitro Cancer Cell Imaging[J]. Chinese Journal of Luminescence, 2015, 36(9): 989.

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