光子学报, 2015, 44 (10): 1017001, 网络出版: 2015-11-30  

荧光猝灭分析中内滤效应的精确校正

Method of Accurate Correction on Inner Filter Effects in Fluorescence Quenching Analysis
作者单位
合肥工业大学 电子科学与应用物理学院,合肥 230009
摘要
研究了荧光猝灭分析中荧光强度的演变过程,发现样品对激发光的吸收分布会伴随内滤效应对荧光强度产生潜在影响.根据吸收分布影响荧光强度的物理机制,提出了利用荧光响应函数去除吸收分布影响的校正方法.通过分析五聚噻吩与富勒烯C70之间在不同激发波长处光致电子转移引起的荧光猝灭,对校正效果进行验证,结果显示:单独对内滤效应进行校正后,各激发波长处的荧光猝灭率与C70的吸收谱仍具有弱相关性,进一步对吸收分布的影响加以校正,所得荧光猝灭率则不再与C70的吸收谱关联.表明在荧光猝灭分析时增加对吸收分布的校正可有效提高内滤效应的最终校正准确度.
Abstract
Via investigation on the evolution of fluorescence intensity in quenching process,it is discovered that the absorption distribution of exciting light can influence the fluorescence intensity in a potential way when the inner filter effects occur. According to the impact mechanism of absorption distribution on fluorescence intensity,a correction method based on fluorescence response function for removing the influence of absorption distribution is proposed. Photo induced fluorescence quenching of Quinquethiophene / fullerene (C70) at different excitation wavelength is tested to inspect the correction effect. Experimental results demonstrate these quenching efficiencies obtained without correction on the influence of absorption distribution weakly correlated with the absorption spectrum of C70,while the corrected ones are independent of them. Which means further correction on absorption distribution can effectively improve the final correction accuracy in fluorescence quenching analysis.

陈向东, 袁自钧, 高峰, 高伟清. 荧光猝灭分析中内滤效应的精确校正[J]. 光子学报, 2015, 44(10): 1017001. CHEN Xiang-dong, YUAN Zi-jun, GAO Feng, GAO Wei-qing. Method of Accurate Correction on Inner Filter Effects in Fluorescence Quenching Analysis[J]. ACTA PHOTONICA SINICA, 2015, 44(10): 1017001.

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