碱性橙Ⅱ是一种非食用物质, 可能被非法用于食品染色。 本文以十六烷基三甲基溴化铵（CTAB）为表面活性剂, 采用化学合成法制备了均匀的纳米金表面增强拉曼散射活性基底, 建立了灵敏快速检测碱性橙Ⅱ的拉曼光谱检测方法。 运用多元变量分析方法验证各浓度之间存在显著性差异, 选取碱性橙Ⅱ在1 594 cm-1处的拉曼特征峰, 单变量分析表明拉曼散射强度与碱性橙Ⅱ浓度的对数呈线性相关, 线性范围0.001 mmol·L-1 ～ 0.5 mmol·L-1, 相关系数r=0.990 2。 将本法应用于腐竹中碱性橙Ⅱ的测定, 在添加浓度为50和500 μg·g-1时, 添加回收率为82.4%～116.9 %, 相对标准偏差为3.8%～4.0%。 与常规化学分析技术相比, 本法具有无损、 快速、 环保、 消耗化学试剂少、 所需样品量少等特点, 适于对大规模样品进行筛查。

Chrysoidin is a kind of banned food dye, and it has been illegally used for coloring food. A rapid detection and quantification method is developed and applied in analysis chrysoidin in yuba. Gold nanoparticles are synthesized by using hexadecyl trimethyl ammonium bromide (CTAB) as the bifunctional ligand to link the solid substrate and the AuNPs. The laser wavelength used for quantitative is 1 594 cm-1. Significant differences between different concentrations of chrysoidin are verified by multiple variable analysis. A relationship between the logarithm of the concentrations and the intensity of laser is proved using univariate analysis method. The calibration curves showed good linearity in the range of 0.001～0.5 mmol·L-1 with correlation coefficients r=0.995. The method is successfully applied to the determination of chrysoidin in yuba. The average recoveries of the drugs spiked at 50 and 500 μg·g-1 levels are 82.4% and 116.9%, and the relative standard deviations (RSD) are 3.8% and 4.0%. The method is simple, rapid, sensitive and accurate in the determination of chrysoidin.