在传统荧光光谱技术的基础上, 结合金属纳米颗粒的增强荧光技术, 探索提高荧光光谱技术检测人全血溶液中胆固醇含量的精度和分辨率的方法。 实验研究方面, 采用波长为407 nm的激光作为激发光, 照射加入一定量银纳米颗粒作为荧光增强剂的人全血溶液, 研究了银纳米颗粒对人全血溶液在可见光波段的荧光增强作用。 结果表明, 胶体状态的银纳米颗粒可以显著增强低浓度的人全血溶液荧光光谱的强度, 且不同位置荧光发射峰的荧光增强效率随银胶加入量的增加均呈现先增后减的趋势, 但不同峰位置的最强增强效率对应的银胶加入量不同。 理论分析方面, 根据实验结果及胆固醇分子和银纳米颗粒在溶液中的分布情况, 建立了分子间距模型, 并根据模型计算得出胆固醇分子和银纳米粒子之间的最佳增强荧光效果间距在12.19～25 nm范围内, 这个结果和其他文献中的理论值吻合较好。 综上所述, 使用银纳米颗粒可实现全血溶液荧光的增强, 研究结果为提高检测血液中多种物质的灵敏度和精度提供了有价值的参考作用。

Based on traditional fluorescence spectroscopy and metal nanoparticles-enhanced fluorescence technology, this research explores a method of improving the accuracy and resolution of cholesterol detected by fluorescence spectroscopy in human whole blood solution. In experiment, an adult blood with silver nanoparticles is radiated by a laser pulse with wavelength of 407 nm, the fluorescence enhancement effect of cholesterol in blood is studied. The results show that, colloidal silver nanoparticles can enhance the fluorescence intensity of cholesterol in human blood with low concentration significantly. With the increase of the amount of silver colloids, the enhanced efficiency of fluorescence peaks at different positions increases first, and then decreases. However, the strongest enhanced efficiency of fluorescence peaks is different corresponding to different amount of silver colloids. According to the experimental results and the distribution of cholesterol molecules and silver nanoparticles in solution, molecular spatial distribution model is established by theoretical analyses, and the optimal distance for efficient fluorescence enhancement between cholesterol molecules and silver nanoparticles is calculated, the range is 12.19～25 nm, and the result is in good agreement with the theoretical values in other literatures. In summary, the fluorescence intensity of cholesterol in human blood can be enhanced by colloidal silver nanoparticles, and the results also provide a valuable reference on improving the sensitivity and accuracy of cholesterol detection.