显微共聚焦拉曼光谱成像技术(Confocal Raman Microspectroscopy Imaging, CRMI)能够对样品微区进行精确无损的拉曼光谱分析和光谱图像扫描, 提供生物样品的无损高分辨光学信息。本项研究工作, 利用CRMI技术实验获取了正常人体离体皮肤组织的拉曼光谱特征, 并结合典型特征峰的扫描图像, 探讨了脂类、蛋白质等成分在皮肤真皮层的分布特点。实验发现皮肤组织真皮层内胶原蛋白的拉曼特征峰1 248 cm-1强度及其空间分布尤为突出, 这一实验结果与组织学中胶原纤维占真皮结缔组织95%的事实相符。实验结果显示, CRMI技术能够全面诠释生物组织内部生化组成与分布信息, 在实验描述皮肤组织病理变化的分子生物学机制方面具有广阔的应用前景。

Confocal Raman Microspectroscopy Imaging (CRMI) provides a precise tool to illustrate biochemical nature and structure of biological tissue without introducing any external labels. It enables a high-contrast and high-resolution imaging of molecular composition by reconstructing selected Raman bands of each spectrum obtained from every scanning points on the sample. In this work, CRMI was utilized to investigate Raman features of ex vivo human normal skin tissue, and visualize the lipids and protein distribution pattern in skin dermis layer. The acquired spectral data showed that the prominent spectral features is the major vibration bands around 1 248 cm-1 assigned to collagen content in dermis, which have a good correlation with skin histology fact that collagen fiber account for 95% of the dermal connective tissue. The achieved results exhibit that spectra-structure correlation can be clearly addressed by tissue biochemical profiles with CRMI method. The results also indicate that CRMI have a potentially broad application on studying molecular pathogenesis in skin diseases.