研究了D5S818、D8S1179、D7S820、CSF1PO、D2S1338、D3S1358、vWA、D21S11、D16S539、PentaE、TPOX、TH01、D19S433、D18S51、FGA、D6S1043、D13S317、D12S391等18个短串联重复序列(short tandem repeats, STR)基因座在甘肃地区回族人群的遗传多态性。采用荧光标记复合扩增及毛细管电泳技术对1 038名甘肃地区回族无关个体18个STR基因座进行分析。研究结果显示, 1 038名甘肃地区回族个体在18个STR基因座上, 共检出223种等位基因, 982种基因型, 其分布均符合Hardy-Weinberg平衡(P>0.05), 18个基因座的杂合度(H)介于0.601～0.929之间, 匹配概率(Pm)介于0.012~0.213之间, 个体识别概率(DP)介于0.787～0.988之间, 多态信息含量(PIC)介于0.550～0.920之间, 非父排除概率(PE)值介于0.292~0.854之间。本文研究结果对甘肃地区回族人群群体遗传学及法医学后续研究应用具有参考价值。

This study was to determine the frequencies and parameters of Hui population 18 short tandem repeat loci (D5S818、D8S1179、D7S820、CSF1PO、D2S1338、D3S1358、vWA、D21S11、D16S539、PentaE、TPOX、TH01、D19S433、D18S51、FGA、D6S1043、D13S317、D12S391) in Gansu region. PCR amplification and capillary electrophoresis technologies were employed to determine the genetype of 18 STR loci for 1 038 unrelated Hui individuals. Results show that 223 alleles and 982 genotypes are recognized in Hui population from Gansu region. The results demonstrated that all loci are found to have no deviation from Hardy-Weinberg equilibrium(P>0.05). Heterozygosity(H) ranged from 0.601 to 0.929, matching probability(Pm) ranged from 0.012 to 0.213, power of discrimination(DP) ranged from 0.787 to 0.988, polymorphism information content(PIC) ranged from 0.550 to 0.920, power of exclusion(PE) ranged from 0.292 to 0.854. The research results of this work have reference value for the research and application of the population genetics and forensic DNA of Hui population from Gansu region.