木质纤维类生物质水解过程中产生的可溶性木素、 呋喃类化合物在紫外波长范围内均有吸收峰, 且吸收峰相互叠加。 研究提出了一种基于紫外光谱法快速测定生物质水解液中糠醛（F）和5-羟甲基糠醛（5-HMF）混合物（M）的方法。 研究发现, M中5-HMF的含量百分率（W5-HMF）和最大吸收波长（λmax）存在良好的线性关系, R2=0.998 4。 进一步研究表明M中W5-HMF不变时, 混合物浓度（cM）和吸光度值（A）之间也存在良好的线性关系, R2在0.998 6～1.000 0之间, 且重现性均良好。 因此, 基于A, cM及λmax, W5-HMF之间的良好线性关系, 可以准确地检测出F和5-HMF的含量, 其相对偏差、 回收率及RSD分别为5.6%和3.7%, 89%～96%, 0.21%～0.85%。 该方法简单、 快捷、 准确, 适应于乙醇法精炼木质纤维类生物质水解液中F和5-HMF混合物的测定。

The soluble lignin and furan compounds in hydrolysate of lignocellulose biomass have absorbed peaks and inter-superimpose in UV spectroscopy. Therefore, this paper developed a method for the determination of furfural and 5-hydroxymethyl furfural in ethanol-water refining liquors of lignocellulose biomass based on UV spectroscopy. The study showed that there is a good and linear relationship between maximum absorption (λmax) and the concentration percent of 5-HMF (W5-HMF) of M, the regression coefficient up to 0.998 4 (R2=0.998 4). Furthermore, a better linear relationship was also observed between the concentration of mixture (cM) and absorptivity (A) when the W5-HMF was constant, the R2 of 0.998 6～1.000 0 and reproducibility are desirable. The contents of F and 5-HMF could be calculated accurately according to the perfect liner relationship of A and cM, λmax and W5-HMF, and the relative deviation of F and 5-HMF of 5.6% and 3.7%, recovery of 89%～96%, RSD of 0.21%～0.85%, respectively. Overall, The simple rapid and precise method in this paper can be applied to the determination of the F and 5-HMF in the hydrolysate of ethanol-water refining lignocellulose biomass.