光学学报, 2017, 37 (5): 0518001, 网络出版: 2017-05-05
小型光谱编码显微成像系统的研制
Development of Compact Spectrally Encoded Microscopy Imaging System
摘要
光谱编码显微镜使用一个衍射光栅和光谱分析装置来获得显微图像。样品上不同的位置被不同的波长照明, 通过对反射光光谱进行解码来得到空间信息。研制了一个基于扫频光源和平衡探测器的小型光谱编码显微镜(CSEM) 。在没有放大器的情况下, 一个固定增益的平衡探测器被用来探测较弱的样品光。通过对一个1951USAF分辨率测试靶成像来测量系统的横向分辨率。对离体猪小肠组织和在体手指皮肤成像来验证生物组织成像性能。结果表明, CSEM具有对生物组织深度分辨成像的能力。
Abstract
Spectrally encoded microscopy uses a diffraction grating and a spectrum analysis setup to obtain microscopic images. The different positions on the sample are illuminated by different wavelengths. Then the spatial information is obtained by decoding the spectrum of reflection light. In this paper, a compact spectrally encoded microscopy (CSEM) is developed based on a swept source and a balanced detection. A fixed gain balanced detector is employed in the system to detect the weak sample light without amplifier. The lateral resolution of the system is measured by imaging a 1951USAF resolution test target. The imagings of excised swine small intestine and the finger skin in vivo are obtained to verify the imaging performance of biological tissue. The results show that the CSEM has the capability of generating depth-resolved imaging of biological tissue.
廖九零, 高万荣. 小型光谱编码显微成像系统的研制[J]. 光学学报, 2017, 37(5): 0518001. Liao Jiuling, Gao Wanrong. Development of Compact Spectrally Encoded Microscopy Imaging System[J]. Acta Optica Sinica, 2017, 37(5): 0518001.