鉴于传统生物芯片检测装置结构较复杂,共聚焦逐点扫描速度较慢,设计了一种使用激光光源、二维高速振镜和冷却型CCD相结合的新装置,并且建立相应的检测数学模型。新装置以STM32为控制核心,采用弓字型扫描方式采集载玻片上的荧光信号。实验对同一片载玻片上的Cy5荧光染料进行五个不同位置的扫描,并由冷却型CCD曝光采集图片。经实验得到了五个相应位置的灰度图像,通过公式推导建立了检测数学模型并用其进行图像灰度校正,校正后的图像灰度误差在2%以内,可以满足光强一致性的要求。

Since the scanning speed of the confocal device is slow and structure of the traditional biological chip detection device is complex,we design a new device with laser light source,two-dimensional high speed vibrating mirrors and a cooling CCD camera.Further,a corresponding mathematical test model was developed.The proposed device uses STM32 as the control chip and collects fluorescent signal by scanning the micro-slide in an arched direction.In the experiment,cyanine(Cy5) dye dots in the same micro-slide were scanned in the five different positions.Furthermore,pictures were collected by the cooling CCD camera.The results show that gray images obtained in the five different positions were corrected by detection mathematic model.In addition,gray correction error was within 2%,which satisfies the requirement of light intensity consistency.