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多色双光子成像技术进展

Recent Advances in Multicolor Two-Photon Imaging Technique

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摘要

双光子荧光显微成像是一种非线性光学显微技术,具有高空间分辨率、高信噪比和固有的三维层析分辨能力等优点。传统的双光子荧光显微成像通常使用波长可调谐的100 fs超短脉冲激光器作为激光光源。目前,人们对双光子荧光显微成像方法进行了深入研究,改进光源及探测方法是常用的手段。介绍和总结了多色双光子荧光显微成像技术的近期研究进展及其在生物医学中的应用。首先介绍了传统飞秒激光器及光学参量振荡器在多色成像中的应用,然后对光纤超连续谱在多色显微成像中的应用进行了分析,最后简要说明了增强自相位调制效应产生连续光谱以及选择性激发实现多色成像的工作。多色双光子成像技术不仅可以同时获取含有多种荧光团的待测样品的高对比度双光子荧光图像,而且具有系统结构简单、操作简便等优点,这使得其在生物医学和材料科学等领域具有广阔的应用前景,并且为生物医学诊断与研究提供了一种有效的工具和平台。

Abstract

Two-photon fluorescence microscopy is a kind of nonlinear optical microscopy, which has the advantages of high time resolution, spatial resolution, high signal to noise ratio and intrinsic three-dimensional ability. Traditional two-photon fluorescence microscopy generally adopts tunable ultra-short pulse laser with pulsewidth of 100 fs as the light source. At present, two-photon fluorescence microscopy is further studied, and light source and detection method improvements are common means. In this paper, we introduce and summarize the recent advances in multicolor two-photon excited fluorescence microscopy and its application in biomedicine. Firstly, traditional application of femtosecond laser and optical parametric oscillator in multicolor imaging is introduced. Secondly, we focus on the application of fiber supercontinuum in multi-color microscopic imaging. Finally, we make a description of our recent work on continuum spectrum generation by enhanced self-phase modulation and selective excitation to achieve multicolor imaging. Multicolor two-photon imaging technology has the advantages of simple set-up, simple operation, and it has been broadly applied in biomedical and material science. Which provides a powerful tool and platformfor biomedical diagnosis and research.

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中图分类号:R318.51

DOI:10.3788/lop54.060002

所属栏目:综述

基金项目:国家自然科学基金(61522502)

收稿日期:2016-11-25

修改稿日期:2017-01-23

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作者单位    点击查看

崔 权:华中科技大学武汉光电国家实验室(筹)Britton Chance生物医学光子学研究中心, 湖北 武汉 430074华中科技大学生物医学工程系生物医学光子学教育部重点实验室, 湖北 武汉 430074
陈忠云:华中科技大学武汉光电国家实验室(筹)Britton Chance生物医学光子学研究中心, 湖北 武汉 430074华中科技大学生物医学工程系生物医学光子学教育部重点实验室, 湖北 武汉 430074
张智红:华中科技大学武汉光电国家实验室(筹)Britton Chance生物医学光子学研究中心, 湖北 武汉 430074华中科技大学生物医学工程系生物医学光子学教育部重点实验室, 湖北 武汉 430074
骆清铭:华中科技大学武汉光电国家实验室(筹)Britton Chance生物医学光子学研究中心, 湖北 武汉 430074华中科技大学生物医学工程系生物医学光子学教育部重点实验室, 湖北 武汉 430074
付 玲:华中科技大学武汉光电国家实验室(筹)Britton Chance生物医学光子学研究中心, 湖北 武汉 430074华中科技大学生物医学工程系生物医学光子学教育部重点实验室, 湖北 武汉 430074

联系人作者:崔权(D201177657@hust.edu.cn)

备注:崔 权(1989-),男,博士研究生,主要从事光纤连续光谱及双光子荧光显微成像方面的研究。

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引用该论文

Cui Quan,Chen Zhongyun,Zhang Zhihong,Luo Qingming,Fu Ling. Recent Advances in Multicolor Two-Photon Imaging Technique[J]. Laser & Optoelectronics Progress, 2017, 54(6): 060002

崔 权,陈忠云,张智红,骆清铭,付 玲. 多色双光子成像技术进展[J]. 激光与光电子学进展, 2017, 54(6): 060002

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