光谱学与光谱分析, 2017, 37 (6): 1745, 网络出版: 2017-07-10
基于2D-COS红外光谱的附子炮制过程时序段解析研究
Research on Steaming Procedure of Aconite Root with FTIR and Two-Dimensional IR Correlation Spectroscopy
附子 红外光谱 二阶导数谱 二维相关谱 时序段 Aconite tuber Infrared spectroscopy Two-dimensional correlation spectroscopy Steaming procedure Time sequence
摘要
采用傅里叶变换红外光谱技术, 获得蒸制过程中附子红外光谱及二维相关谱(Tow-dimensional correlation, 2D-COS), 研究附子炮制过程特征。 分别比较原始红外光谱、 二阶导数谱及二维相关谱, 结果表明红外原始光谱图十分相似, 结合二阶导数光谱分析1 63487 cm-1为羰基峰; 1 60318, 1 57158, 1 48509和1 41308 cm-1为苯环的骨架振动峰, 85141和75924 cm-1 为苯环C—H键的振动峰; 1 15373, 1 08184和1 02135 cm-1为生物碱中酯键和醇羟基的特征峰。 二维相关谱结果揭示附子在炮制过程中发生的2个过程时序段, 分别为2~3和8~9 h。 研究数据有助于临床根据适应证选择适宜的煎煮时间, 避免煎煮不足导致中毒或煎煮太过影响疗效。
Abstract
Fourier transform infrared spectroscopy (FTIR), second derivative infrared spectroscopy, and two-dimensional correlation infrared spectroscopy (2D-IR) were used to study the steaming procedure of aconite tuber. The results showed that samples with steaming procedures were similar to each other in one-dimensional IR spectra but different in 2D-IR spectra. The FTIR spectra combined with second derivative infrared spectroscopy of different samples during boiling procedure present characteristic peaks at 1 63487 cm-1 (CO), 1 60318, 1 57158 cm-1, 1 48509, 1 41308 cm-1 (vibration of phenyl framework) and 1 15373, 1 08184, 1 02135 cm-1 (C—O) respectively. There were two conspicuous time sequences in two dimensional infrared correlation spectroscopy of autopeaks intensity. Since, the intensity of autopeaks was relatively high during 2~3 and 8~9 h. The function of FTIR and 2D-IR for analysis of the alkaloid from aconite root attributed high speed and high efficiency. The results were reliable and valuable.
吴志生, 刘晓娜, 谭鹏, 裴艳玲, 戴幸星, 乔延江. 基于2D-COS红外光谱的附子炮制过程时序段解析研究[J]. 光谱学与光谱分析, 2017, 37(6): 1745. WU Zhi-sheng, LIU Xiao-na, TAN Peng, PEI Yan-ling, DAI Xing-xing, QIAO Yan-jiang. Research on Steaming Procedure of Aconite Root with FTIR and Two-Dimensional IR Correlation Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2017, 37(6): 1745.