采用重复性及生物兼容性较好的二维纳米银膜作为氧合血红蛋白表面增强拉曼散射光谱的(SERS)基底, 利用具有较好匹配的近红外激光作为激发光源, 通过引起共振拉曼效应分别对健康女性和女性乳腺癌患者的氧合血红蛋白进行SERS光谱研究。比较两组氧合血红蛋白平均SERS光谱, 发现存在差异。利用SPSS软件的主成分分析(PCA)方法和独立变量T检验统计分析方法, 发现两组光谱在659、813和1 122 cm-1拉曼频移处的谱峰有着极显著的区别。对主成分进行归属分析, 女性乳腺癌患者氧合血红蛋白分子中, 吡咯环的反对称变形振动、对称变形振动以及吡咯环的呼吸振动的强度、相对于健康女性显著减少, 从而造成图谱谱峰有明显差异。除此以外, SERS技术结合SPSS统计分析方法能够较好地区分健康女性和乳腺癌患者的氧合血红蛋白, 从而有望发展成为一种新型的乳腺癌临床诊断技术。

Based on the repeatability and good biological compatibility two-dimensional nano silver membrane as oxyhemoglobin near infrared surface enhanced Raman scattering(SERS), a study on oxyhemoglobin used SERS spectra was carried out by using near infrared laser with good match as excitation light source, which caused resonance Raman effect respectively of healthy women and women with breast cancer. The study found that obvious differences existed between healthy women and female breast cancer patients′ oxyhemoglobin. By using the method of principal component analysis(PCA) and independent variable T test statistical analysis through SPSS, an extremely significant difference was found between the two spectra in 659, 813 and 1122 cm-1 Raman shift spectrum peak. By attribution analysis on the principal component, a significant reduction was found, compared with the healthy women, in the female breast cancer patients′ oxygenated hemoglobin molecules, pyrrole ring vibration, and symmetric deformation of antisymmetric deformation and pyrrole ring breathing vibration intensity, which resulted in an obvious difference in the graph spectral peaks. In addition, SERS technique can be combined with SPSS statistical analysis method to distinguish the oxygenated hemoglobin between healthy women and patients with breast cancer, which is expected to develop into a new type of clinical diagnosis technology for breast cancer.