激光生物学报, 2017, 26 (3): 260, 网络出版: 2017-07-07   

不同方法检测鸡蛋表面菌落总数的相关性研究

A Study on the Correlation of Different Methods for Detecting the Total Number of Bacteria on the Surface of Eggs
作者单位
1 湖南农业大学食品科技学院, 湖南 长沙410128
2 湖南农业大学食品科技学院, 湖南 长沙 410128
3 食品科技和生物技术湖南省重点实验室, 湖南 长沙 410128
摘要
研究了ATP生物荧光检测法与国标法《GB4789.2-2010食品卫生微生物学检验菌落总数测定》检测鸡蛋壳表面细菌总数的相关性。采用ATP生物荧光检测法和国标法对40个混合样品表面细菌总数进行检测, 以logCFU/个蛋壳为横坐标(x), 以logRLU/个蛋壳为纵坐标(y), 分别进行线性、对数、乘幂、指数拟合。结果表明, ATP荧光检测法与国标法检测结果Pearson相关系数为0.912, 线性模型y=0.7306x-1.0041(R2=0.8322)拟合度较高。该试验结果为ATP荧光检测法在鸡蛋壳表面细菌总数快速检测中应用的可行性提供了依据。
Abstract
In this study, the bacterial count of the egg shell surface was compared by two methods: GB4789.2-2010 microbiological examination of food Hygiene: detection of aerobic bacterial count, ATP bioluminescence assay to study the correlation between ATP count and routine bacterial detection. The total bacteria count on the surface of 40 mixed samples was detected by ATP fluorescence detection, and the national standard method was used as the control. The Pearson correlation coefficient of ATP fluorescence test and the result of national standard method was 0.912. On the basis of these results, with log (CFU) as abscissa (x) and log (RLU) as ordinate (y), the linear fitting, logarithm fitting and exponent fitting were conducted. The fitting degree logarithmic model y=0.7306x-1.0041(R2=0.8322) was higher. ATP fluorescence detection method and the national standard method in the egg shell surface bacterial load detection results have a good correlation. ATP fluorescence detection can be replaced to some extent, routine testing of bacteria and applied to the actual production.

程教擘, 李梦丹, 刘金, 谢艳华, 陈力力, 刘焱. 不同方法检测鸡蛋表面菌落总数的相关性研究[J]. 激光生物学报, 2017, 26(3): 260. CHENG Jiaobo, LI Mengdan, LIU Jin, XIE Yanhua, CHEN Lili, LIU Yan. A Study on the Correlation of Different Methods for Detecting the Total Number of Bacteria on the Surface of Eggs[J]. Acta Laser Biology Sinica, 2017, 26(3): 260.

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