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Inversion Method of Plant Photosynthesis Parameter Based on Fast Phase and Relaxation Fluorescence Kinetics

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According to the biological film energy flow theory and electron transfer model, the plant complex photosynthesis is analyzed and simplified by combining to the exciting conditions of fast phase and relaxation, so that an inversion method of plant photosynthesis parameter is proposed .The maximum fluorescence yield is calculated by using the determinate method of sliding window slope. The photochemical quantum efficiency and the functional absorption cross section are obtained by analyzing the fast phase fluorescence process with linear least square algorithm. The plastoquinone average reduction time constant is obtained by analyzing the relaxation fluorescence process with discrete iterative algorithm. The experimental measurement results of the merismopedia and scenedesmu sobliquus grew in logarithmic phase and under the copper ion stress conditions show that the inversion has good stability and repeatability. The relative standard deviation of the photochemical quantum efficiency, the functional absorption cross section and the plastoquinone average reduction time constant are 1.25%, 1.50% and 1.83%, respectively. The linear correlation coefficient of the photochemical efficiency compared to the measuring result of the pulse amplitude modulation technology is 0.9714. The proposed inversion method provides an optical analysis means for the study of plant physiology.





基金项目:国家自然科学基金(31400317)、国家863计划 (2014AA06A509)、国家重点研发计划项目(2016YFC1400602)、安徽省科技重大专项(15CZZ04125)、安徽省自然科学基金青年项目(1708085QD87)、青岛海洋科学与技术国家实验室开发基金项目(QNLM2016ORP0312)




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覃志松:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031中国科学技术大学 安徽 合肥 230026桂林电子科技大学计算机与信息安全学院, 广西 桂林 541004
赵南京:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031
殷高方:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031
石朝毅:合肥学院电子工程系, 安徽 合肥 230601
甘婷婷:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031
肖 雪:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031
段静波:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031
张小玲:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031中国科学技术大学 安徽 合肥 230026
陈 双:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031中国科学技术大学 安徽 合肥 230026
刘建国:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031
刘文清:中国科学院安徽光学精密机械研究所安徽省环境光学监测技术重点实验室, 安徽 合肥 230031



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Qin Zhisong,Zhao Nanjing,Yin Gaofang,Shi Chaoyi,Gan Tingting,Xiao Xue,Duan Jingbo,Zhang Xiaoling,Chen Shuang,Liu Jianguo,Liu Wenqing. Inversion Method of Plant Photosynthesis Parameter Based on Fast Phase and Relaxation Fluorescence Kinetics[J]. Acta Optica Sinica, 2017, 37(7): 0730002

覃志松,赵南京,殷高方,石朝毅,甘婷婷,肖 雪,段静波,张小玲,陈 双,刘建国,刘文清. 快相与弛豫荧光动力学植物光合作用参数反演方法[J]. 光学学报, 2017, 37(7): 0730002

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