光子学报, 2017, 46 (7): 0722003, 网络出版: 2017-08-09
激光扫描光学断层成像系统的设计与应用
The Design of Scanning Laser Optical Tomography Imaging System and Its Applications
激光扫描光学断层成像 荧光成像 介观尺寸 生物器官 三维信息 成像系统 Scanning laser optical tomography Fluorescence microscopy Mesoscopic size LabVIEW LabVIEW Biological organs Three-dimensional information Imaging systems
摘要
设计了基于LabVIEW控制的激光扫描光学断层成像系统, 该系统以准直激光器为光源, 高精度四维平移台为样本定位单元, 光电倍增管为采集单元, 实现了针对小鼠肺等小尺寸样本的三维成像.该系统空间分辨率优于20 μm, 成像视野大于1 cm, 通过对离体小鼠肺器官的自发荧光成像, 展示了系统的操作流程、成像结果和初步生物应用, 并探索了它对整个生物器官组织的成像能力.与传统生物医学光学成像方法相比, 该方法具有光子收集效率高、成像样本尺寸大、系统操作简单等优点.
Abstract
This paper presented a self-designed scanning laser optical tomography mesoscopic imaging system based on LabVIEW and explored its capabilities to image intact biological organs or tissues. The system is consisted of an aligned laser, a four dimension translation stage, a scanning mirror and a photomultiplier tube. The spatial resolution of the system is better than 20 μm with an imaging field of 1 cm. The system operation process and preliminary biomedical applications were demonstrated through the 3-D autofluorescence imaging of a mouse lung. Compared to the traditional biomedical optical imaging techniques, scanning laser optical tomography provides the advantages of high photon collection efficiency, large sample range and easy operation.
薛成志, 李亚敏, 李贵叶, 李颖超, 胡学娟, 陈玲玲. 激光扫描光学断层成像系统的设计与应用[J]. 光子学报, 2017, 46(7): 0722003. XUE Cheng-zhi, LI Ya-min, LI Gui-ye, LI Ying-chao, HU Xue-juan, CHEN Ling-ling. The Design of Scanning Laser Optical Tomography Imaging System and Its Applications[J]. ACTA PHOTONICA SINICA, 2017, 46(7): 0722003.