肿瘤一直威胁着人类的健康, 大多数肿瘤患者死于转移性瘤而非原发性瘤。循环肿瘤细胞(CTC)是肿瘤转移的重要标志, CTC的检测可以用来监控肿瘤的转移情况及预后评估。在体光声流式细胞术(PAFC)利用CTC与血液背景的光吸收差异可以实现CTC的在体检测。相比于传统的CTC检测方法, PAFC可以无创、高灵敏地检测循环系统中的CTC数目。总结了PAFC无标记检测黑色素瘤CTC信号及纳米颗粒靶向标记乳腺癌CTC信号的方法, 以及这些方法在肿瘤转移、治疗效果评估中的应用。

Tumor is a great threat to human health, the majority of tumor patients die from metastatic tumor rather than primary tumor. Circulating tumor cell (CTC) is an important indication of metastatic tumor, and its detection can be used for monitoring tumor metastasis and prognosis evaluation. Based on the difference of light absorption between CTC and blood background, in vivo photoacoustic flow cytometry (PAFC) can realize CTC in vivo detection. Compared with the traditional CTC detection methods, the advantage of PAFC is that it can detect the number of CTC in the circulatory system, and the detection is noninvasive and high sensitive. This review summarizes the methods of the real-time and label-free detection of melanoma CTC and the real-time detection of breast cancer by nanoparticle targeting, and their application in evaluation of tumor metastasis and treatment effect.