中国激光, 2017, 44 (10): 1007001, 网络出版: 2017-10-18
白细胞对偏振光的散射特性及散射特性与细胞结构的关系 下载: 953次
Scattering Characteristics of Leukocytes on Polarized Light and Relationship Between Scattering Characteristics and Cell Structure
生物光学 细胞分析 光散射 偏振光 穆勒矩阵 几何光学近似 biotechnology cell analysis light scattering polarized light Müller matrix geometric optics approximation
摘要
为了研究白细胞对偏振光的散射特性, 运用几何光学近似理论修正偏振光传输的斯托克斯-穆勒矩阵元, 基于偏心球模型数值模拟了两束偏振化方向互相垂直的偏振光入射时, 不同形状、大小、折射率的无粒白细胞的散射光强空间分布以及散射特性与细胞结构的关系。不同条件下偏振光散射三维分布图像呈现不同的条纹特征, 表明分布规律与细胞模型的形体参量和光学参量具有相关性。散射光强的差值、比值、差和比的分析结果表明, 偏振光的后向散射反映了细胞内部更丰富的结构信息和光学信息。
Abstract
To study the scattering characteristics of leukocytes at polarized light incidence, we modify Stokes-Müller matrix elements for polarized light propagation by using the geometric optics approximation theory. On the basis of eccentric sphere model, we numerically simulate the scattering light intensity spatial distribution of the mitochondria free leukocytes with different shapes, sizes and refractive indices with two polarized beams incident, and polarization directions of the two beams are perpendicular to each other. The relationship between the scattering characteristic and cell structure parameters, such as shape, size, and refractive index, is numerically simulated. The three-dimensional distribution of polarized light scattering shows different fringe features under different conditions, which illustrates that the distribution is relevant to physical parameters and optical parameters. The analysis of differences, ratios, and ratios of difference to sum of the scattering intensity shows that the backscattering of the polarized light can reflect rich structural information and optical information in the cell.
卜敏, 胡双双, 陶兆禾, 金卫凤, 王亚伟. 白细胞对偏振光的散射特性及散射特性与细胞结构的关系[J]. 中国激光, 2017, 44(10): 1007001. Bu Min, Hu Shuangshuang, Tao Zhaohe, Jin Weifeng, Wang Yawei. Scattering Characteristics of Leukocytes on Polarized Light and Relationship Between Scattering Characteristics and Cell Structure[J]. Chinese Journal of Lasers, 2017, 44(10): 1007001.