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水凝胶支架的飞秒激光全息加工

Femtosecond laser holograpic fabrication of hydrogel cell scaffold

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摘要

为了实现以水凝胶为材料的细胞支架快速加工, 将计算全息法引入传统的飞秒激光双光子加工中, 并对全息图的生成方法和全息图对加工结构的影响进行了研究。首先, 根据贝塞尔光波动方程和其透射函数生成贝塞尔光束全息图, 分析两种参数对环形结构大小和质量的影响。然后利用生成的全息图加工得到壁厚800 nm、直径为8~15 μm不等的水凝胶(PEGDA)圆管结构。最后, 实现了基于圆管道的水凝胶细胞支架高效快速加工, 支架中圆管道壁厚800 nm、直径为8 μm。本文首次将飞秒激光全息加工技术应用于水凝胶三维支架加工, 解决了飞秒激光单点加工效率的问题。该技术在生物医学中具有广阔的应用前景。

Abstract

In order to achieve rapid fabrication of hydrogel cell scaffolds, the computer-aided holographic method was employed into the traditional femtosecond laser two-photon processing. The generation of hologram as well as the influence of its parameters on the processed cylinder microstructure was studied. Firstly, Ring-shaped Bessel-beam holograms were generated according to the Bessel light wave equation and its transmission function, of which the parameters effect on the size and quality of ring structure was analyzed. Then the generated holograms were used to process tubes with a thickness of 0.8 μm and diameters from 8 μm to 15 μm in the polyethylene glycol double acrylate (PEGDA) hydrogel. Finally, an efficient and rapid processing of hydrogel cell scaffolds was realized experimentally and the diameter of hydrogel tubes was 8 μm. This technique can remarkably improve the efficiency of femtosecond laser processing, thus has great potential in many applications such as biological culture and chemical analyses.

Newport宣传-MKS新实验室计划
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中图分类号:TN249;TH691.9

DOI:10.3788/ope.20172509.2277

所属栏目:现代应用光学

基金项目:国家自然科学基金资助项目(No.51675503,No.61475149,No.51405464,No.61675190,No.51605463); 中央高校基本研究经费资助项目(No.WK2480000002); 中国博士后科学基金资助项目(No.2016M590578,No.2016M602027); 中科院仪器研制项目(No.YZ201566)

收稿日期:2017-06-05

修改稿日期:2017-06-27

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作者单位    点击查看

潘 登:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026
李家文:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026
杨 亮:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026
胡衍雷:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026
吴 东:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026
黄文浩:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026
褚家如:中国科学技术大学 精密机械与精密仪器系, 安徽 合肥 230026

联系人作者:潘登(dpan@mail.ustc.edu.cn)

备注:潘 登(1996-), 男, 安徽六安人, 2012年于北京科技大学获得学士学位, 主要从事飞秒激光加工和光场调控方面的研究。

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引用该论文

PAN Deng,LI Jia-wen,YANG Liang,HU Yan-lei,WU Dong,HUANG Wen-hao,CHU Jia-ru. Femtosecond laser holograpic fabrication of hydrogel cell scaffold[J]. Optics and Precision Engineering, 2017, 25(9): 2277-2282

潘 登,李家文,杨 亮,胡衍雷,吴 东,黄文浩,褚家如. 水凝胶支架的飞秒激光全息加工[J]. 光学 精密工程, 2017, 25(9): 2277-2282

被引情况

【1】顾银炜,陈 达,李久荣,董建峰,王 琴,王 刚,陶卫东. 飞秒激光双光子聚合构建水溶性石墨烯-光刻胶的3D微结构. 红外与激光工程, 2018, 47(10): 1006006--1

【2】李 源,宋寰宇,张 韵,牛 佳,刘博文,胡明列. 光谱调制对飞秒脉冲自相似放大系统的影响. 红外与激光工程, 2019, 48(1): 103005--1

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